Pereira Cristiano G, Silva Alexandre L N, de Castilhos Patrícia, Mastrantonio Eneida C, Souza Richard A, Romão Raquel P, Rezende Renata J, Pena Janethe D O, Beletti Marcelo E, Souza Maria A
Laboratório de Biologia Molecular, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Brazil.
Vet Parasitol. 2009 Nov 12;165(3-4):231-40. doi: 10.1016/j.vetpar.2009.07.019. Epub 2009 Jul 19.
The aim of this study was to evaluate the histopathological features in tissues of mice infected by human isolates (I, II, and III) or the reference M2903 strain of Leishmania braziliensis complex. BALB/c and C57Bl/6 mice were infected in the hind footpad with 10(6) stationary-phase promastigotes of L. braziliensis complex. The evolution of lesions was observed for 10 weeks and the animals were then euthanized and liver, spleen and popliteal lymph nodes were collected. Tissues were stained with hematoxylin and eosin and analyzed by immunohistochemistry assay. Increased thickness of infected footpads was observed in all animals, lesions were nodular and non-ulcerated. Mice infected with isolate I presented inflammatory infiltrates consisting predominantly of mononuclear cells in all tissues examined, and also a great number of megakaryocytes, compared with other isolates. Infection with isolate II led to an infected footpad enlargement not seen in other isolates. In addition, mononuclear infiltrates in the liver and hemosiderin in spleen were noted. Conversely, mice infected with either isolate III or M2903 strain only showed an increased number of megakaryocytes in spleen. All tissues examined had detectable amastigote forms of Leishmania by immunohistochemistry in all groups. Taking together, our results showed an unforeseen behavior of different isolates of L. braziliensis complex that led to diverse pathological findings.
本研究的目的是评估感染人类分离株（I、II和III）或巴西利什曼原虫复合体参考M2903菌株的小鼠组织中的组织病理学特征。将BALB/c和C57Bl/6小鼠的后足垫用10(6)个巴西利什曼原虫复合体的静止期前鞭毛体进行感染。观察病变的演变10周，然后对动物实施安乐死并收集肝脏、脾脏和腘淋巴结。组织用苏木精和伊红染色，并通过免疫组织化学分析。在所有动物中均观察到感染足垫厚度增加，病变为结节状且未溃疡。与其他分离株相比，感染分离株I的小鼠在所有检查的组织中均出现主要由单核细胞组成的炎性浸润，并且还有大量巨核细胞。感染分离株II导致感染足垫肿大，这在其他分离株中未见。此外，在肝脏中观察到单核浸润，在脾脏中观察到含铁血黄素。相反，感染分离株III或M2903菌株的小鼠仅在脾脏中显示巨核细胞数量增加。通过免疫组织化学在所有组的所有检查组织中均检测到利什曼原虫的无鞭毛体形式。综上所述，我们的结果显示巴西利什曼原虫复合体不同分离株的意外行为导致了不同的病理结果。
