Federal Institute for Risk Assessment, Berlin, Germany.
J Clin Microbiol. 2009 Oct;47(10):3147-55. doi: 10.1128/JCM.00900-09. Epub 2009 Aug 5.
The multiple-locus variable-number repeat analysis of 90 human Brucella melitensis isolates from a large urban area in central Peru revealed variations at 4 (Bruce07, Bruce09, Bruce18, and Bruce42) out of 16 loci investigated, of which 1 (Bruce42) also is used for species identification. Ten genotypes were identified, separated by the number of Bruce42 repeats into two groups that may have distinct phenotypic characteristics. Whereas genotypes with five or six Bruce42 repeats were cultured mainly from adult patients, genotypes with three Bruce42 repeats were isolated from children and young adolescents as well as from adults. In addition, the isolates with three Bruce42 repeats were obtained more often from patients with splenomegaly (P = 0.02) or hepatomegaly (P = 0.006). An annual variation in the diversity of genotypes was observed, possibly reflecting changes in sources of fresh dairy products, supply routes to city shops and markets, and the movement of infected dairy goat herds.
对来自秘鲁中部一个大城市的 90 株人类布鲁氏菌分离株进行多位点可变数重复分析，在 16 个调查的基因座中发现了 4 个（Bruce07、Bruce09、Bruce18 和 Bruce42）发生了变异，其中 1 个（Bruce42）也用于物种鉴定。鉴定出了 10 种基因型，根据 Bruce42 重复的数量将它们分为两组，这两组可能具有不同的表型特征。Bruce42 重复 5 或 6 次的基因型主要从成年患者中培养出来，而 Bruce42 重复 3 次的基因型则从儿童和青少年以及成年患者中分离出来。此外，Bruce42 重复 3 次的分离株更常从脾肿大（P = 0.02）或肝肿大（P = 0.006）的患者中获得。观察到基因型多样性的年度变化，这可能反映了新鲜乳制品的来源、供应路线到城市商店和市场以及受感染的奶山羊群的流动的变化。
