Ishige Ikuo, Nagamura-Inoue Tokiko, Honda Masaki J, Harnprasopwat Ratanakanit, Kido Michiko, Sugimoto Mitsuhiro, Nakauchi Hiromitsu, Tojo Arinobu
Department of Cell Processing and Transfusion, Institute of Medical Science, University of Tokyo, Shirokanedai 4-6-1, Minato-ku, Tokyo, 108-8639, Japan.
Division of Stem Cell Therapy, Center for Stem Cell and Regenerative Medicine, The Institute of Medical Science, University of Tokyo, Shirokanedai 4-6-1, Minato-ku, Tokyo, 108-8639, Japan.
Int J Hematol. 2009 Sep;90(2):261-269. doi: 10.1007/s12185-009-0377-3. Epub 2009 Aug 6.
We isolated mesenchymal stem cells (MSC) from arteries (UCA), veins (UCV), and Wharton's jelly (UCWJ) of human umbilical cords (UC) and determined their relative capacities for sustained proliferation and multilineage differentiation. Individual UC components were dissected, diced into 1-2 mm(3) fragments, and aligned in explant cultures from which migrating cells were isolated using trypsinization. Preparations from 13 UCs produced 13 UCWJ, 11 UCV, and 10 UCA cultures of fibroblast-like, spindle-shaped cells negative for CD31, CD34, CD45, CD271, and HLA-class II, but positive for CD13, CD29, CD44, CD73, CD90, CD105, and HLA-class I. UCV cells exhibited a significantly higher frequency of colony-forming units fibroblasts than did UCWJ and UCA cells. Individual MSCs could be selectively differentiated into osteoblasts, chondrocytes, and adipocytes. When compared for osteogenic potential, UCWJ cells were the least effective precursors, whereas UCA-derived cells developed alkaline phosphatase activity with or without an osteogenic stimulus. UC components, especially blood vessels, could provide a promising source of MSCs with important clinical applications.
我们从人脐带（UC）的动脉（UCA）、静脉（UCV）和华通氏胶（UCWJ）中分离出间充质干细胞（MSC），并确定了它们持续增殖和多向分化的相对能力。将单个UC组件解剖，切成1-2立方毫米的碎片，并在外植体培养物中排列，通过胰蛋白酶消化从中分离出迁移的细胞。来自13条UC的制备物产生了13个UCWJ、11个UCV和10个UCA培养物，培养出的成纤维细胞样、梭形细胞对CD31、CD34、CD45、CD271和HLA-II类呈阴性，但对CD13、CD29、CD44、CD73、CD90、CD105和HLA-I类呈阳性。UCV细胞形成集落形成单位成纤维细胞的频率明显高于UCWJ和UCA细胞。单个MSC可以选择性地分化为成骨细胞、软骨细胞和脂肪细胞。在比较成骨潜力时，UCWJ细胞是最无效的前体细胞，而UCA来源的细胞在有或没有成骨刺激的情况下都会产生碱性磷酸酶活性。UC组件，尤其是血管，可为具有重要临床应用的MSC提供有前景的来源。
