Sumita Yoshinori, Honda Masaki J, Ohara Takayuki, Tsuchiya Shuhei, Sagara Hiroshi, Kagami Hideaki, Ueda Minoru
Tooth Regeneration, Division of Stem Cell Engineering, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
Biomaterials. 2006 Jun;27(17):3238-48. doi: 10.1016/j.biomaterials.2006.01.055. Epub 2006 Feb 28.
Tooth structure can be regenerated by seeding dissociated tooth cells onto polyglycolic acid fiber mesh, although the success rate of tooth production is low. The present study was designed to compare the performance of collagen sponge with polyglycolic acid fiber mesh as a 3-D scaffold for tooth-tissue engineering. Porcine third molar teeth at the early stage of crown formation were enzymatically dissociated into single cells, and the heterogeneous cells were seeded onto collagen sponge or the polyglycolic acid fiber mesh scaffolds. Scaffolds were then cultured to evaluate cell adhesion and ALP activity in vitro. An in vivo analysis was performed by implanting the constructs into the omentum of immunocompromised rats and evaluating tooth production up to 25 weeks. After 24h, there were a significantly higher number of cells attached to the collagen sponge scaffold than the polyglycolic acid fiber mesh scaffold. Similarly, the ALP activity was significantly higher for the collagen sponge scaffold was than the polyglycolic acid fiber mesh scaffold after 7 days of culture. The area of calcified tissue formed in the collagen sponge scaffold was also larger than in the polyglycolic acid fiber mesh scaffold. The results from in vivo experiments show conclusively that a collagen sponge scaffold allows tooth production with a higher degree of success than polyglycolic acid fiber mesh. Taken together, the results from this study show that collagen sponge scaffold is superior to the polyglycolic acid fiber mesh scaffold for tooth-tissue engineering.
通过将解离的牙齿细胞接种到聚乙醇酸纤维网上，牙齿结构可以再生，尽管牙齿生成的成功率较低。本研究旨在比较胶原海绵与聚乙醇酸纤维网作为牙齿组织工程三维支架的性能。将处于牙冠形成早期的猪第三磨牙用酶解离成单细胞，然后将这些异质细胞接种到胶原海绵或聚乙醇酸纤维网支架上。接着对支架进行培养，以评估体外细胞黏附及碱性磷酸酶（ALP）活性。通过将构建体植入免疫缺陷大鼠的大网膜并评估长达25周的牙齿生成情况来进行体内分析。24小时后，附着在胶原海绵支架上的细胞数量明显多于聚乙醇酸纤维网支架。同样，培养7天后，胶原海绵支架的ALP活性也明显高于聚乙醇酸纤维网支架。胶原海绵支架中形成的钙化组织面积也大于聚乙醇酸纤维网支架。体内实验结果确凿表明，与聚乙醇酸纤维网相比，胶原海绵支架在牙齿生成方面成功率更高。综上所述，本研究结果表明，在牙齿组织工程中，胶原海绵支架优于聚乙醇酸纤维网支架。
