Kenyon R H, Condie R M, Jahrling P B, Peters C J
Pathogenesis and Immunology Department, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702-5011.
Microb Pathog. 1990 Oct;9(4):219-26. doi: 10.1016/0882-4010(90)90010-n.
Antibody-containing plasma from patients recovered from Argentine hemorrhagic fever (AHF) is of proven value in treatment of the acute disease, but the possibility of transmitting blood-borne organisms such as HIV and hepatitis virus detracts from this approach. Purified human immune plasma fractions IgG1,2,4, IgG1,2,3,4 and F(ab')2 neutralized Junin virus in vitro. IgG1,2,3,4 and IgG1,2,4 lysed (in the presence of complement) cells infected with Junin virus, and protected infected guinea pigs from AHF. However, large quantities of the immune F(ab')2 fraction from the same plasma pool failed to protect guinea pigs from death, to increase the mean time to death, and to diminish virus load in target organs of infected guinea pigs. This suggests that elimination of infected cells rather than virus neutralization may play a critical role in protection against Junin virus.
从阿根廷出血热（AHF）康复患者体内获取的含抗体血浆在治疗该急性疾病方面已被证明具有价值，但传播血源性病原体（如HIV和肝炎病毒）的可能性降低了这种治疗方法的可行性。纯化的人免疫血浆组分IgG1、2、4，IgG1、2、3、4和F(ab')2在体外可中和胡宁病毒。IgG1、2、3、4和IgG1、2、4（在补体存在的情况下）可裂解感染胡宁病毒的细胞，并保护感染的豚鼠免受AHF侵害。然而，来自同一血浆池的大量免疫F(ab')2组分未能保护豚鼠免于死亡、延长平均死亡时间，也未能降低感染豚鼠靶器官中的病毒载量。这表明消除感染细胞而非病毒中和可能在抵抗胡宁病毒的过程中起关键作用。
