Improved preparative separation of human B and T lymphocytes and determination of relative mobilities of B and T lymphoblast cell lines by free flow electrophoresis.

The improved quantitative separation of human B and T lymphocytes by free flow electrophoresis is presented. Buffer systems with different compositions are tested analytically and partly preparatively for their effect on the separation profile. The best result is achieved with a modified phosphate-buffered saline and allows the enrichment of unstimulated, functionally intact B and T lymphocytes as well as monocytes in three distinct fractions. Furthermore, the electrophoretic mobilities of six established acute lymphoblastic leukemia cell lines are determined with regard to the mobilities of the healthy cells.