Baier T G, Weber G, Hartmann K, Heinrich U, Schönberg D
Department of Pediatrics, University of Heidelberg, Federal Republic of Germany.
Anal Biochem. 1988 May 15;171(1):91-5. doi: 10.1016/0003-2697(88)90127-3.
An electrophoretic method for the quantitative separation of human B and T lymphocytes in a carrier-free system is presented. The method is based on the fact that B and T lymphocytes show marked overlap in their size and density characteristics, but differ sufficiently in surface charge to be separable by electrophoresis. The technique is performed in phosphate-buffered saline and appears to be especially suitable for the enrichment of nonstimulated, functionally intact lymphocytes which can be directly used for further immunological or biochemical studies.
本文介绍了一种在无载体系统中定量分离人B淋巴细胞和T淋巴细胞的电泳方法。该方法基于以下事实：B淋巴细胞和T淋巴细胞在大小和密度特征上有明显重叠，但表面电荷差异足以通过电泳分离。该技术在磷酸盐缓冲盐溶液中进行，似乎特别适用于富集未受刺激、功能完整的淋巴细胞，这些淋巴细胞可直接用于进一步的免疫学或生化研究。
