Zhang Haifang, Sheng Xiumei, Xu Shungao, Gao Yulin, Du Hong, Li Junrong, Xu Huaxi, Huang Xinxiang
Department of Biochemistry and Molecular Biology, School of Medical Technology, Jiangsu University, Jiangsu, China.
FEMS Microbiol Lett. 2009 Sep;298(1):51-5. doi: 10.1111/j.1574-6968.2009.01692.x. Epub 2009 Jun 26.
Recent studies have shown that flagella may modulate physiological processes by sensing environmental changes in temperature and moisture. When the z66(+) strain of Salmonella enterica serovar Typhi (S. Typhi) was exposed to an antiserum against the z66 flagellar antigen, the fljBA operon was deleted from a linear plasmid, leading to the unidirectional flagellar phase variation from FljB to FliC. We hypothesized that flagella may serve as a sensor that responded to the antiserum by altering gene expression and triggering the unidirectional flagellar phase variation. To test this hypothesis, Salmonella genomic DNA microarrays were used to determine the gene expression profile of the z66(+) wild-type strain of S. Typhi treated with the anti-z66 antiserum for 30 min. The results showed that expression levels of 187 genes were altered by more than threefold compared with the same strain treated with control serum. The microarray expression patterns of representative genes were validated by reverse transcriptase-PCR. Importantly, no significant changes in gene expression were observed in the fljB:z66 deletion mutant that was similarly treated with the anti-z66 antiserum. To the best of our knowledge, this is the first study to show the global transcriptional response of Salmonella to antiflagellin antiserum.
最近的研究表明，鞭毛可能通过感知温度和湿度等环境变化来调节生理过程。当肠炎沙门氏菌伤寒血清型（伤寒杆菌）的z66(+)菌株暴露于针对z66鞭毛抗原的抗血清时，fljBA操纵子从线性质粒中缺失，导致鞭毛单向相变，从FljB变为FliC。我们假设鞭毛可能作为一种传感器，通过改变基因表达和触发单向鞭毛相变来对抗血清做出反应。为了验证这一假设，我们使用沙门氏菌基因组DNA微阵列来确定用抗z66抗血清处理30分钟的伤寒杆菌z66(+)野生型菌株的基因表达谱。结果表明，与用对照血清处理的同一菌株相比，187个基因的表达水平变化超过三倍。代表性基因的微阵列表达模式通过逆转录-聚合酶链反应得到验证。重要的是，在用抗z66抗血清进行类似处理的fljB:z66缺失突变体中未观察到基因表达的显著变化。据我们所知，这是第一项展示沙门氏菌对抗鞭毛蛋白抗血清的全局转录反应的研究。
