对1959年至2006年期间在中国分离出的伤寒沙门氏菌菌株进行基因分型，并通过基因组微阵列分析遗传多样性。
Genotyping of Salmonella enterica serovar Typhi strains isolated from 1959 to 2006 in China and analysis of genetic diversity by genomic microarray.
作者信息
Zhang Haifang, Zhang Xiaolei, Yan Meiying, Pang Bo, Kan Biao, Xu Huaxi, Huang Xinxiang
机构信息
Department of Biochemistry and Molecular Biology, School of Basic Medicine and Medical Technology, Jiangsu University, Xuefu road 301, Zhenjiang, China.
出版信息
Croat Med J. 2011 Dec 15;52(6):688-93. doi: 10.3325/cmj.2011.52.688.
AIM
To determine the genotype of Salmonella enterica serovar Typhi (S. Typhi) strains in China and analyze their genetic diversity.
METHODS
We collected S. Typhi strains from 1959 to 2006 in five highly endemic Chinese provinces and chose 40 representative strains. Multilocus sequence typing was used to determine the genotypes or sequence types (ST) and microarray-based comparative genomic hybridization (M-CGH) to investigate the differences in gene content among these strains.
RESULTS
Forty representative S. Typhi strains belonged to 4 sequence types (ST1, ST2, ST890, and ST892). The predominant S. Typhi genotype (31/40) was ST2 and it had a diverse geographic distribution. We discovered two novel STs - ST890 and ST892. M-CGH showed that 69 genes in these two novel STs were divergent from S. Typhi Ty2, which belongs to ST1. In addition, 5 representative Typhi strains of ST2 isolated from Guizhou province showed differences in divergent genes.
CONCLUSION
We determined two novel sequence types, ST890 and ST892, and found that ST2 was the most prevalent genotype of S. Typhi in China. Genetic diversity was present even within a highly clonal bacterial population.
目的
确定中国伤寒沙门氏菌（伤寒杆菌）菌株的基因型，并分析其遗传多样性。
方法
我们收集了1959年至2006年期间中国五个伤寒高发省份的伤寒杆菌菌株，并选取了40株具有代表性的菌株。采用多位点序列分型法确定基因型或序列型（ST），并利用基于微阵列的比较基因组杂交技术（M-CGH）研究这些菌株之间基因含量的差异。
结果
40株具有代表性的伤寒杆菌菌株属于4种序列型（ST1、ST2、ST890和ST892）。主要的伤寒杆菌基因型（31/40）为ST2，且具有广泛的地理分布。我们发现了两种新的序列型——ST890和ST892。M-CGH显示，这两种新序列型中的69个基因与属于ST1的伤寒杆菌Ty2不同。此外，从贵州省分离出的5株具有代表性的ST2伤寒杆菌菌株在差异基因方面也存在不同。
结论
我们确定了两种新的序列型ST890和ST892，发现ST2是中国伤寒杆菌最常见的基因型。即使在高度克隆的细菌群体中也存在遗传多样性。
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