Soltani Jalal, van Heusden Gerard Paul H, Hooykaas Paul J J
Institute of Biology, Leiden University, Leiden, The Netherlands.
FEMS Microbiol Lett. 2009 Sep;298(2):228-33. doi: 10.1111/j.1574-6968.2009.01723.x. Epub 2009 Jul 13.
Agrobacterium tumefaciens is a plant pathogen that genetically transforms plant cells by transferring a part of its Ti-plasmid, the T-strand, to the host cell. Under laboratory conditions, it can also transform cells from many different nonplant organisms, including the yeast Saccharomyces cerevisiae. Collections of S. cerevisiae strains have been developed with systematic deletion of all coding sequences. Here, we used these collections to identify genes involved in the Agrobacterium-mediated transformation (AMT) of S. cerevisiae. We found that deletion of genes (GCN5, NGG1, YAF9 and EAF7) encoding subunits of the SAGA, SLIK, ADA and NuA4 histone acetyltransferase complexes highly increased the efficiency of AMT, while deletion of genes (HDA2, HDA3 and HST4) encoding subunits of histone deacetylase complexes decreased AMT. These effects are specific for AMT as the efficiency of chemical (lithium acetate) transformation was not or only slightly affected by these deletions. Our data are consistent with a positive role of host histone deacetylation in AMT.
根癌土壤杆菌是一种植物病原体，它通过将其Ti质粒的一部分（T链）转移到宿主细胞来对植物细胞进行遗传转化。在实验室条件下，它还能转化许多不同的非植物生物的细胞，包括酿酒酵母。已经构建了系统删除所有编码序列的酿酒酵母菌株文库。在这里，我们利用这些文库来鉴定参与酿酒酵母农杆菌介导转化（AMT）的基因。我们发现，编码SAGA、SLIK、ADA和NuA4组蛋白乙酰转移酶复合物亚基的基因（GCN5、NGG1、YAF9和EAF7）的缺失显著提高了AMT的效率，而编码组蛋白脱乙酰酶复合物亚基的基因（HDA2、HDA3和HST4）的缺失则降低了AMT。这些效应对于AMT是特异性的，因为化学（醋酸锂）转化的效率没有受到这些缺失的影响或仅受到轻微影响。我们的数据与宿主组蛋白去乙酰化在AMT中起积极作用一致。
