TDV 29 Mayis Private Ankara Hospital, Dermatology Clinic, Ankara, Turkey.
Eur J Dermatol. 2009 Nov-Dec;19(6):565-9. doi: 10.1684/ejd.2009.0788. Epub 2009 Aug 6.
Chronic wounds pose important problems in clinical practice and their treatment is difficult and costly. Here we describe a new delayed wound healing animal model. Fifteen male New Zealand rabbits were used in this study. A horizontal incision 4 cm in length was made on the dorsal part of the torso and pure skin flaps were raised in front of and behind this incision. This exposed the panniculus carnosus layer and it was resected. Skin flaps were returned to their places and sutured. After a 3 week period of healing third degree burn injury was inflicted using hot metal plates both on the healed flaps and at the same location on the opposite side. Scar samples were sent for histopathological examination after healing. The wounds on the panniculectomy side healed in an average of 43.20 days but on the control side they healed in an average of 32.80 days (p < 0.05). Wound healing was slower and scars were broader and more irregular on the panniculectomy side. In our new model, addition of panniculectomy to full thickness burn injury significantly delayed wound healing with a decrease in scar quality. This is a simple, economic and effective animal model to study delayed wound healing.
慢性伤口在临床实践中构成重要问题,其治疗既困难又昂贵。在此,我们描述了一种新的延迟性伤口愈合动物模型。本研究共使用 15 只雄性新西兰兔。在躯体背部做一长 4cm 的水平切口,于该切口前后掀起纯皮瓣,暴露腹侧脂肪层并将其切除。皮瓣还纳原位并缝合。愈合 3 周后,用热金属板在愈合皮瓣和对侧同一部位造成三度烧伤。愈合后送检瘢痕组织行组织病理学检查。去脂组创面平均愈合时间为 43.20 天,而对照组为 32.80 天(p<0.05)。去脂组的伤口愈合较慢,瘢痕较宽且不规则。在我们的新模型中,全层烧伤合并去脂术显著延迟了伤口愈合,且瘢痕质量下降。这是一种简单、经济、有效的研究延迟性伤口愈合的动物模型。