Reeves P R, Johnson M A, Holmes I H, Dyall-Smith M L
Department of Microbiology, University of Sydney, NSW, Sydney, Australia.
Res Microbiol. 1990 Sep-Oct;141(7-8):1019-25. doi: 10.1016/0923-2508(90)90144-f.
We have cloned antigenic regions of the VP7 gene from rotavirus RV-5 into the lamB gene. The insertions discussed in this paper comprise 250bp of rotavirus DNA and cover the A and B antigenic regions of the protein. The fusion proteins are expressed and are present in the outer membrane: they react with anti-rotavirus antibody. However, as yet we have not been able to demonstrate that the fusion has LamB protein functions with regard to maltodextrins or L phage, and the fusion protein appears to exist in the outer membrane as a monomer rather than as a trimer.
