Maldonado A J, Bastardo J W
Departamento de Biología, Escuela de Ciencias, Universidad de Oriente, Cumaná, Venezuela.
Acta Cient Venez. 1992;43(6):368-72.
During the period January to December 1987, rotaviruses were obtained from infants hospitalized with acute gastroenteritis in Cumaná, Venezuela, and were compared by analysis of the migration of their double-stranded ribonucleic acid (RNA) genome segments in polyacrylamide gel electrophoresis. Rotavirus RNA was detected in 87 (17.03%) of 511 faecal samples, and at least 12 different electropherotypes were recognized throughout the period of study. Of the rotavirus positive samples, 86 (98.85%) had a "long" electrophoretic pattern characteristic of human rotavirus subgroup 2. The incidence of rotavirus infection was statistically related with the age, sex, and nutritional status of the pediatric patients for epidemiological purposes. This statistical relation was very significant with the age and the nutritional status of the patients but not with their sex.
在1987年1月至12月期间,从委内瑞拉库马纳因急性肠胃炎住院的婴儿中获取轮状病毒,并通过分析其双链核糖核酸(RNA)基因组片段在聚丙烯酰胺凝胶电泳中的迁移情况进行比较。在511份粪便样本中,87份(17.03%)检测到轮状病毒RNA,在整个研究期间至少识别出12种不同的电泳型。在轮状病毒阳性样本中,86份(98.85%)具有人类轮状病毒2型亚组特有的“长”电泳模式。出于流行病学目的,轮状病毒感染的发生率与儿科患者的年龄、性别和营养状况存在统计学关联。这种统计学关联在患者年龄和营养状况方面非常显著,但与患者性别无关。