Foschi D, Castoldi L, Radaelli E, Abelli P, Calderini G, Rastrelli A, Mariscotti C, Marazzi M, Trabucchi E
Department of Surgery, Institute of Biomedical Sciences L. Sacco, Milan, Italy.
Int J Tissue React. 1990;12(6):333-9.
Oxygen free-radicals are known to impair wound healing after ischaemia-reperfusion or polymorphonuclear cell stimulation. Furthermore, they reduce the breaking strength of all recent wounds and might be a cause of wound leakage. This study was performed to evaluate whether or not hyaluronic acid can reduce the risk of wound impairment caused by free-radicals, in rats with abdominal sepsis, polymorphonuclear cell stimulation or cytochrome C function derangement produced by xenobiotics. Male Sprague-Dawley rats with open wounds received phenazine methosulfate or zimosan, or had abdominal sepsis to induce oxygen free-radical generation. There were three groups of treatment: hyaluronic acid cream, hyaluronic acid ethyl ester gel, and placebo. The reduction in wound size was measured from the 1st to the 11th postoperative day; biopsies were taken for histological evaluation. Every other day, a gentle debridement was performed in all the groups of animals. We found that hyaluronic acid and its ethyl ester derivative significantly improved the wound healing of rats subjected to an increased generation of oxygen free-radicals. It remains to be established whether or not hyaluronic acid acts as a scavenger of free-radicals.
已知氧自由基会损害缺血再灌注或多形核细胞刺激后的伤口愈合。此外，它们会降低所有近期伤口的抗张强度，可能是伤口渗漏的一个原因。本研究旨在评估透明质酸是否能降低腹部脓毒症、多形核细胞刺激或外源性物质引起的细胞色素C功能紊乱的大鼠中由自由基导致的伤口损伤风险。有开放性伤口的雄性Sprague-Dawley大鼠接受吩嗪硫酸甲酯或酵母聚糖，或患有腹部脓毒症以诱导氧自由基生成。有三组治疗方法：透明质酸乳膏、透明质酸乙酯凝胶和安慰剂。从术后第1天到第11天测量伤口大小的减小情况；取组织活检进行组织学评估。每隔一天，对所有动物组进行轻柔的清创。我们发现透明质酸及其乙酯衍生物显著改善了氧自由基生成增加的大鼠的伤口愈合。透明质酸是否作为自由基清除剂还有待确定。
