Yi Lun-zhao, Yuan Da-lin, Liang Yi-zeng, Xie Pei-shan, Zhao Yu
Research Center of Modernization of Chinese Medicines, Central South University, Changsha 410083, China.
Anal Chim Acta. 2009 Sep 1;649(1):43-51. doi: 10.1016/j.aca.2009.07.009. Epub 2009 Jul 8.
Tangerine peels are herbal materials of two coupled traditional Chinese medicines, Pericarpium Citri Reticulatae (PCR) and Pericarpium Citri Reticulatae Viride (PCRV). In this paper, high-performance liquid chromatographic fingerprints of tangerine peels during growth were firstly measured for deliberately collected 34 samples from three species (Citrus reticulata 'Chachi', Citrus reticulata 'Dahongpao' and Citrus erythrosa Tanaka). After sixteen characteristic components which have similar change trends in the grown process were screened out with the help of heuristic evolving latent projection (HELP) method, score plots of principal component analysis (PCA) successfully presented the grown footprints of tangerine peels. It implied that July might be the best harvest time for PCRV, November and December were better for PCR. Furthermore, hesperidin, nobiletin and tangeretin were screened as chemical markers by loadings of PCA. The HPLC-HELP-PCA strategy has shown its potential in optimization of harvest time and chemical markers' screening, which will have wide perspective in the analysis of "coupled TCMs".
陈皮是两种相关联的传统中药——陈皮（PCR）和青皮（PCRV）的药材。本文首次对生长过程中的陈皮进行了高效液相色谱指纹图谱测定，特意从三种柑橘（茶枝柑、大红袍和朱橘）中采集了34个样品。借助启发式演化潜投影（HELP）方法筛选出在生长过程中具有相似变化趋势的16种特征成分后，主成分分析（PCA）得分图成功呈现了陈皮的生长轨迹。这表明7月可能是青皮的最佳采收时间，11月和12月对陈皮而言采收较好。此外，通过PCA载荷筛选出橙皮苷、川陈皮素和橘皮素作为化学标志物。HPLC-HELP-PCA策略在采收时间优化和化学标志物筛选方面显示出了潜力，这在“关联中药”分析中将具有广阔前景。
