Lin Le-wei, Jiang Lin, Zheng Guo-dong
School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China.
Zhong Yao Cai. 2010 Feb;33(2):173-6.
To determine the contents of hesperidin, nobiletin and tangeretin in Citrus reticulata 'Chachi' from various habitats and different collecting periods (from October to December) and study the dynamic change of three flavonoids constituents.
The HPLC method was used for analysis the contents of flavonoids in Citrus reticulata 'Chachi'. The system used a Diamonsil C18 column (250 mm x 4.6 mm, 5 microm) with gradient mobile phase of acetonitrile-methanol (80:20)-2% acetic acid. The monitoring wavelength was at 283 nm and 330 nm and the column temperature was at 25 degrees C with the flow rate of 1.0 ml/min.
The contents of hesperidin, nobiletin and tangeretin in Citrus reticulata 'Chachi' collecting from various habitats descended gradually with the mature of fruit, especially in nobiletin and tangeretin.
The method was simple, convenient and can be used to provide some foundation for the quality control of Citrus reticulata 'Chachi'.
测定不同产地及不同采收期（10月至12月）的茶枝柑中橙皮苷、川陈皮素和橘皮素的含量，并研究这三种黄酮类成分的动态变化。
采用高效液相色谱法分析茶枝柑中黄酮类成分的含量。该系统使用迪马C18柱（250 mm×4.6 mm，5 µm），流动相为乙腈 - 甲醇（80:20）-2%乙酸进行梯度洗脱。监测波长为283 nm和330 nm，柱温为25℃，流速为1.0 ml/min。
不同产地采收的茶枝柑中，橙皮苷、川陈皮素和橘皮素的含量随着果实成熟逐渐下降，尤其是川陈皮素和橘皮素。
该方法简便易行，可为茶枝柑的质量控制提供一定依据。
