Fu Manqin, Xu Yujuan, Chen Yulong, Wu Jijun, Yu Yuanshan, Zou Bo, An Kejing, Xiao Gengsheng
Sericultural & Agri-Food Research Institute Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, PR China.
Sericultural & Agri-Food Research Institute Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, PR China.
Food Chem. 2017 Sep 1;230:649-656. doi: 10.1016/j.foodchem.2017.03.098. Epub 2017 Mar 18.
A simple and accurate method using high performance liquid chromatography (HPLC) with dual wavelength detection was developed to simultaneously determine the contents of one flavanone glycoside (hesperidin) and five polymethoxylated flavones (PMFs: sinensetin, 4',5,7,8-tetramethoxyflavone, nobiletin, tangeretin and 5-O-desmethyl nobiletin) in Pericarpium Citri Reticulatae (Citrus reticulata 'Chachi') ('Chachi' PCR). By modifying the mobile phase compositions and detection wavelengths, an optimal HPLC condition was obtained, under which the calibration curves of all six compounds exhibited good linearity (R>0.99). For all the tested compounds, the relative standard deviation (RSD) was less than 4%, and the accuracy ranged from 97.58 to 103.2%. The developed method was successfully applied to monitor the changes in the contents of six flavonoids in 'Chachi' PCR during storage at 25°C, over a three year period. Color parameters and antioxidant capacity were also determined to evaluate the sample quality. The contents of hesperidin decreased while all the polymethoxylated flavones and antioxidant activities increased throughout the storage period, demonstrating that polymethoxylated flavones could be used as indices for the quality change of Chachi' PCR during storage. The results from this study suggest that the longer storage periods increased the quality of PCR.
建立了一种采用高效液相色谱（HPLC）双波长检测的简单准确方法，用于同时测定茶枝柑（Citrus reticulata 'Chachi'）果皮（'Chachi' PCR）中一种黄烷酮苷（橙皮苷）和五种多甲氧基黄酮（PMFs：川陈皮素、4',5,7,8-四甲氧基黄酮、诺米林、橘红素和5-O-去甲基诺米林）的含量。通过调整流动相组成和检测波长，获得了最佳的HPLC条件，在此条件下，所有六种化合物的校准曲线均呈现良好的线性关系（R>0.99）。对于所有测试化合物，相对标准偏差（RSD）小于4%，准确度在97.58%至103.2%之间。所建立的方法成功应用于监测'Chachi' PCR在25°C下储存三年期间六种黄酮类化合物含量的变化。还测定了颜色参数和抗氧化能力以评估样品质量。在整个储存期间，橙皮苷含量下降，而所有多甲氧基黄酮含量和抗氧化活性均增加，表明多甲氧基黄酮可作为'Chachi' PCR储存期间质量变化的指标。本研究结果表明，储存时间越长，PCR的质量越高。
