Li Ruijun, Gao Yanqi, Hou Yulin, Ye Shigen, Wang Lianshun, Sun Jingxian, Li Qiang
Agriculture Department Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Dalian Key Laboratory of Marine Animal Disease Control and Prevention, Dalian Ocean University, Dalian, China.
Department of Ocean Technology, College of Marine and Biology Engineering, Yancheng Institute of Technology, Yancheng, China.
Mitochondrial DNA B Resour. 2018 Jul 3;3(2):736-737. doi: 10.1080/23802359.2018.1483757.
Scuticociliates are dangerous parasitic pathogens for in worldwide mariculture. Scuticociliates cause high mortality to marine fish. After an outbreak of scuticociliatosis in in Liaoning Province, northern China, , a scuticociliate, was identified. In this study, using Illumina MiSeq next-generation sequencing, the mitochondrial genome of was assembled and analysed phylogenetically using mitochondrial genomes of other scuticociliates. The complete mitochondrial genome was 39,845 bp; it contained two rRNAs, six tRNAs, and 39 protein-coding genes (PCGs). From the 39 PCGs, 15 PCGs were located on the heavy strand, and 24 PCGs on the light strand of mitogenome. The phylogenetic tree showed that there were two main clades, Oligohymenophorea and Spirotrichea. Nine ciliate species were clustered together within Oligohymenophorea; was a separate cluster sharing a relatively close ancestry with Hymenostomatida. The results of this study will help advance the systematics, and studies of evolution and molecular epidemiology of scuticociliates.
盾纤毛虫是全球海水养殖中危险的寄生性病原。盾纤毛虫会导致海水鱼类的高死亡率。在中国北方辽宁省爆发盾纤毛虫病后，一种盾纤毛虫被鉴定出来。在本研究中，利用Illumina MiSeq下一代测序技术，组装了该盾纤毛虫的线粒体基因组，并使用其他盾纤毛虫的线粒体基因组进行了系统发育分析。该盾纤毛虫的完整线粒体基因组为39,845 bp；它包含两个rRNA、六个tRNA和39个蛋白质编码基因（PCG）。在这39个PCG中，15个PCG位于重链上，24个PCG位于该线粒体基因组的轻链上。系统发育树显示有两个主要分支，即寡膜纲和旋毛纲。九个纤毛虫物种在寡膜纲中聚集在一起；该盾纤毛虫是一个单独的聚类，与膜口目有着相对较近的祖先关系。本研究结果将有助于推进盾纤毛虫的系统分类学以及进化和分子流行病学研究。
