Coughlan B M, Moroney G A, van Pelt F N A M, O'Brien N M, Davenport J, O'Halloran J
Environmental Research Institute, University College Cork, Cork, Ireland.
Mar Pollut Bull. 2009 Nov;58(11):1680-4. doi: 10.1016/j.marpolbul.2009.06.020. Epub 2009 Aug 6.
This study investigated the internal osmotic regulatory capabilities of the Manila clam (Ruditapes philippinarum) following in vivo exposure to a range of salinities. A second objective was to measure the health status of the Manila clam following exposure to different salinities using the neutral red retention (NRR) assay, and to compare results using a range of physiological saline solutions (PSS). On exposure to seawater of differing salinities, the Manila clam followed a pattern of an osmoconformer, although they seemed to partially regulate their circulatory haemolytic fluids to be hyperosmotic to the surrounding aqueous environment. Significant differences were found when different PSS were used, emphasizing the importance of using a suitable PSS to reduce additional osmotic stress. Using PSS in the NRR assay that do not exert additional damage to lysosomal membrane integrity will help to more accurately quantify the effects of exposure to pollutants on the organism(s) under investigation.
本研究调查了菲律宾蛤仔（Ruditapes philippinarum）在体内暴露于一系列盐度后其内部的渗透调节能力。第二个目标是使用中性红保留（NRR）试验来测定菲律宾蛤仔在暴露于不同盐度后的健康状况，并使用一系列生理盐溶液（PSS）比较结果。在暴露于不同盐度的海水时，菲律宾蛤仔呈现出渗透顺应者的模式，尽管它们似乎会部分调节其循环溶血流体，使其相对于周围水环境呈高渗状态。当使用不同的PSS时发现了显著差异，这强调了使用合适的PSS以减少额外渗透应激的重要性。在NRR试验中使用不对溶酶体膜完整性造成额外损害的PSS将有助于更准确地量化暴露于污染物对所研究生物体的影响。
