Department of Neurology, College of Medicine, Hallym University, Chunchon 200-702, Kangwon-Do, South Korea.
Brain Res Bull. 2009 Dec 16;80(6):433-42. doi: 10.1016/j.brainresbull.2009.07.016. Epub 2009 Aug 7.
In order to confirm the species-specific distribution of voltage-gated K(+) (Kv) channels and the definitive relationship between their immunoreactivities and seizure activity, we investigated Kv2.x, Kv3.x and Kv4.x channel immunoreactivities in the hippocampi of seizure-resistant (SR) and seizure-sensitive (SS) gerbils. There was no difference in Kv2.1, Kv3.4, Kv4.2 and Kv4.3 immunoreactivity in the hippocampus between SR and SS gerbils. In comparison to SR gerbils, Kv3.1b immunoreactivity in neurons was significantly lower in SS gerbils instead Kv3.1b-immunoreactive astrocytes were clearly observed in SS gerbils (p<0.05). Kv3.2 immunoreactivity was also significantly lower in neurons of SS gerbils than in those of SR gerbils (p<0.05). Considering the findings of our previous study, these findings suggest that delayed rectifier K(+) channels (Kv1.1, Kv1.2, Kv1.5, Kv1.6, Kv2.1 and Kv3.1-2), not A-type K(+) channels (Kv1.4, Kv3.4 and Kv4.x), may be down-regulated in the SS gerbil hippocampus, as compared to SR gerbils.
为了确认电压门控钾通道（Kv）的种属特异性分布以及它们的免疫反应性与癫痫发作活动之间的明确关系，我们研究了癫痫耐药（SR）和癫痫敏感（SS）沙土鼠海马中的 Kv2.x、Kv3.x 和 Kv4.x 通道免疫反应性。在 SR 和 SS 沙土鼠的海马中，Kv2.1、Kv3.4、Kv4.2 和 Kv4.3 的免疫反应性没有差异。与 SR 沙土鼠相比，SS 沙土鼠神经元中的 Kv3.1b 免疫反应性明显降低，而 SS 沙土鼠中清楚地观察到 Kv3.1b 免疫反应性星形胶质细胞（p<0.05）。SS 沙土鼠神经元中的 Kv3.2 免疫反应性也明显低于 SR 沙土鼠（p<0.05）。考虑到我们之前研究的结果，这些发现表明，与 SR 沙土鼠相比，延迟整流钾通道（Kv1.1、Kv1.2、Kv1.5、Kv1.6、Kv2.1 和 Kv3.1-2）而非 A 型钾通道（Kv1.4、Kv3.4 和 Kv4.x）可能在 SS 沙土鼠海马中下调。
