Pierre P, Denis O, Bazin H, Mbongolo Mbella E, Vaerman J P
UCL-ICP-MEXP, Brussels, Belgium.
Eur J Immunol. 1992 Dec;22(12):3179-82. doi: 10.1002/eji.1830221223.
Oral administration to mice of ovalbumin (OVA), if given together with cholera toxin (CT) or its B subunit (CTB) prevented the hyporesponsiveness to OVA subsequently injected parenterally. Oral immunization with CT plus OVA or OVA plus CTB in fact primed the immune system, inducing a stronger response to a subsequent parenteral injection of OVA with complete Freund's adjuvant than in mice prefed only with OVA or with saline. Oral CT plus OVA also induced good serum IgG1 and IgA anti-OVA responses, with slightly (not significant) decreased IgG2a and IgG2b responses. Our in vivo findings agree well with earlier in vitro data from others, including CT inhibition of the Th1 CD4+ T cell subset and with CT effect on B cells (induction of LPS-stimulated IgM+ B cells to undergo increased switch differentiation to IgG1- and IgA-secreting cells).
给小鼠口服卵清蛋白（OVA），若同时给予霍乱毒素（CT）或其B亚基（CTB），可预防随后经肠胃外注射OVA引起的低反应性。事实上，用CT加OVA或OVA加CTB进行口服免疫可启动免疫系统，与仅预先喂食OVA或生理盐水的小鼠相比，对随后用完全弗氏佐剂经肠胃外注射OVA产生更强的反应。口服CT加OVA还可诱导良好的血清IgG1和IgA抗OVA反应，IgG2a和IgG2b反应略有（不显著）降低。我们的体内研究结果与其他人早期的体外数据非常吻合，包括CT对Th1 CD4 + T细胞亚群的抑制作用以及CT对B细胞的作用（诱导LPS刺激的IgM + B细胞增加向分泌IgG1和IgA细胞的类别转换分化）。
