Babbitt Callie W, Pacheco Adriana, Lindner Angela S
School of Human Evolution and Social Change, Arizona State University, P.O. Box 872402, Tempe, AZ 85287-2402, USA.
Bioresour Technol. 2009 Dec;100(24):6207-16. doi: 10.1016/j.biortech.2009.06.110. Epub 2009 Aug 8.
Motivated by the need to establish an economical and environmentally friendly methanol control technology for the pulp and paper industry, a bench-scale activated carbon biofiltration system was developed. This system was evaluated for its performance in removing methanol from an artificially contaminated air stream and characterized for its bacterial diversity over time, under varied methanol loading rates, and in different spatial regions of the filter. The biofilter system, composed of a novel packing mixture, provided an excellent support for growth and activity of methanol-degrading bacteria, resulting in approximately 100% methanol removal efficiency for loading rates of 1-17 g/m(3) packing/h, when operated both with and without inoculum containing enriched methanol-degrading bacteria. Although bacterial diversity and abundance varied over the length of the biofilter, the populations present rapidly formed a stable community that was maintained over the entire 138-day operation of the system and through variable operating conditions, as observed by PCR-DGGE methods that targeted all bacteria as well as specific methanol-oxidizing microorganisms. Phylogenetic analysis of bands excised and sequenced from DGGE gels indicated that the biofilter system supported a diverse community of methanol-degrading bacteria, with high similarity to species in the genera Methylophilus (beta-proteobacteria), Hyphomicrobium and Methylocella (both alpha-proteobacteria).
受为制浆造纸工业建立经济且环保的甲醇控制技术需求的推动，开发了一种实验室规模的活性炭生物过滤系统。对该系统去除人工污染气流中甲醇的性能进行了评估，并对其在不同甲醇负荷率下以及过滤器不同空间区域随时间的细菌多样性进行了表征。由新型填料混合物组成的生物过滤系统为甲醇降解细菌的生长和活性提供了良好的支撑，在接种和未接种富含甲醇降解细菌的接种物的情况下运行时，对于1-17 g/m(3)填料/小时的负荷率，甲醇去除效率约为100%。尽管生物过滤器长度上的细菌多样性和丰度有所不同，但通过针对所有细菌以及特定甲醇氧化微生物的PCR-DGGE方法观察到，存在的菌群迅速形成了一个稳定的群落，该群落在系统整个138天的运行过程中以及不同运行条件下都得以维持。从DGGE凝胶上切下并测序的条带的系统发育分析表明，生物过滤系统支持多种甲醇降解细菌群落，与嗜甲基菌属（β-变形菌）、生丝微菌属和甲基孢囊菌属（均为α-变形菌）中的物种高度相似。
