Abteilung Mikrobiologie, Fachbereich Biologie/Chemie, Universität Osnabrück, Osnabrück, Germany.
Appl Microbiol Biotechnol. 2010 Jan;85(4):1189-99. doi: 10.1007/s00253-009-2290-3. Epub 2009 Oct 22.
The hexane-degrading bacterial community of a biofilter was characterised by a combination of stable isotope-based phospholipid fatty acid analyses, fluorescence in situ hybridisation and cultivation. About 70 bacterial strains were isolated from a full-scale biofilter used for treatment of hexane containing waste gas of an oil mill. The isolation approach led to 16 bacterial groups, which were identified as members of the Alpha-, Beta- and Gammaproteobacteria, Actinobacteria and Firmicutes. Three groups showed good growth on hexane as the sole source of carbon. These groups were allocated to the genera Gordonia and Sphingomonas and to the Nevskia-branch of the Gammaproteobacteria. Actively degrading populations in the filter material were characterised by incubation of filter material samples with deuterated hexane and subsequent phospholipid fatty acid analysis. Significant labelling of the fatty acids 16:1 cis10, 18:1 cis9 and 18:0 10methyl affiliated the hexane-degrading activity of the biofilter with the isolates of the genus Gordonia. In vitro growth on hexane and in situ labelling of characteristic fatty acids confirmed the central role of these organisms in the hexane degradation within the full-scale biofilter.
采用稳定同位素磷脂脂肪酸分析、荧光原位杂交和培养相结合的方法，对生物滤池中的己烷降解细菌群落进行了研究。从用于处理油厂含己烷废气的大型生物滤池中分离出约 70 株细菌。分离方法得到了 16 个细菌群，这些细菌被鉴定为α-、β-和γ-变形菌、放线菌和厚壁菌的成员。有 3 个细菌群可以己烷为唯一碳源良好生长，这些细菌群被分配到戈登氏菌属和鞘氨醇单胞菌属以及γ-变形菌的内斯凯亚分支。通过用氘代己烷孵育滤料样品并随后进行磷脂脂肪酸分析，对滤料中活跃的降解菌群进行了表征。脂肪酸 16:1 cis10、18:1 cis9 和 18:0 10methyl 的显著标记将生物滤池的己烷降解活性与戈登氏菌属的分离物联系起来。在己烷上的体外生长和特征脂肪酸的原位标记证实了这些生物在大型生物滤池中己烷降解过程中的核心作用。
