Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7264, USA.
J Hered. 2010 Jan-Feb;101(1):42-53. doi: 10.1093/jhered/esp066.
Exercise improves many aspects of human health, yet many people remain inactive even when exercise is prescribed. We previously created a backcross (BC) between mice selectively bred for high levels of voluntary wheel running (VWR) and fixed for "mini muscle" (MM), a recessive mutation causing approximately 50% reduction in triceps surae mass. We previously showed that BC mice having the MM trait ran faster and further than mice without MM and that MM maps to chromosome 11. Here, we genotyped the BC with genome-wide single nucleotide polymorphisms to identify quantitative trait loci (QTL) controlling voluntary exercise and tissue and body mass traits and to determine whether these QTL interact with the MM locus or with sex. We detected 3 VWR QTL, representing the first voluntary exercise QTL mapped using this high running selection line, and 5 tissue mass QTL. Several interactions between trait QTL and the MM locus as well as sex were also identified. These results begin to explain the genetic architecture of VWR and further support MM as a locus having major effects, including its main effects on the muscle phenotype, its pleiotropic effects on wheel running and tissue mass traits, and through its interactions with other QTL and with sex.
锻炼可以改善人类健康的许多方面，但即使有运动处方，许多人仍然不运动。我们之前在经过选择性培育高自愿轮跑（VWR）水平和固定“迷你肌肉”（MM）的老鼠之间创建了回交（BC），MM 是一种隐性突变，导致比目鱼肌质量减少约 50%。我们之前表明，具有 MM 特征的 BC 老鼠比没有 MM 的老鼠跑得更快、更远，并且 MM 位于 11 号染色体上。在这里，我们对 BC 进行了全基因组单核苷酸多态性基因分型，以鉴定控制自愿运动以及组织和体重特征的数量性状基因座（QTL），并确定这些 QTL 是否与 MM 基因座或性别相互作用。我们检测到 3 个 VWR QTL，代表使用这条高跑步选择线首次映射的自愿运动 QTL，以及 5 个组织质量 QTL。还鉴定了几个性状 QTL 与 MM 基因座以及性别之间的相互作用。这些结果开始解释 VWR 的遗传结构，并进一步支持 MM 作为具有主要影响的基因座，包括其对肌肉表型的主要影响、对轮跑和组织质量性状的多效性影响，以及通过与其他 QTL 和性别相互作用。
