The Key Laboratory for Microbial Resources of the Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
Xinjiang Institute of Microbiology, Xinjiang Academy of Agricultural Science, Urumqi, Xinjiang 830091, PR China.
Int J Syst Evol Microbiol. 2010 Jun;60(Pt 6):1317-1421. doi: 10.1099/ijs.0.014993-0. Epub 2009 Aug 10.
A Gram-stain-positive actinobacterium, designated strain YIM 93316(T), was isolated from a salt lake in Xinjiang Province, north-west China, and was subjected to a polyphasic taxonomic study. The isolate grew at 10-45 degrees C, at pH 6-9 and in the presence of 1-15 % (w/v) NaCl, but no growth was observed in the absence of NaCl. The cell-wall diamino acid contained alanine, glutamine and lysine with peptidoglycan type A4alpha. Polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, an unknown glycolipid and an unknown phospholipid. The predominant menaquinone was MK-8(H(4)). The major fatty acids were anteiso-C(15 : 0) and anteiso-C(15 : 1). The DNA G+C content of strain YIM 93316(T) was 70.1 mol%. Chemotaxonomic properties supported the affiliation of strain YIM 93316(T) to the genus Georgenia, and this was supported by phylogenetic analysis based on 16S rRNA gene sequences. Levels of 16S rRNA gene sequence similarity between strain YIM 93316(T) and Georgenia thermotolerans TT02-04(T), Georgenia ruanii YIM 004(T) and Georgenia muralis 1A-C(T) were 96.6, 96.5 and 96.3 %, respectively. Data from fatty acid, physiological and biochemical tests allowed the clear phenotypic differentiation of strain YIM 93316(T) from recognized members of the genus Georgenia. On the basis of evidence from the present polyphasic study, strain YIM 93316(T) is considered to represent a novel species of the genus Georgenia, for which the name Georgenia halophila sp. nov. is proposed. The type strain is YIM 93316(T) (=DSM 21365(T) =CCTCC AB 208144(T)).
一株革兰氏阳性放线菌，命名为 YIM 93316(T) 菌株，从中国西北部新疆的一个盐湖中分离得到，并进行了多相分类学研究。该分离株在 10-45°C、pH6-9 和 1-15%（w/v）NaCl 存在下生长，但在没有 NaCl 的情况下不生长。细胞壁二氨基庚二酸含有丙氨酸、谷氨酰胺和赖氨酸，肽聚糖类型为 A4alpha。极性脂含有双磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇、磷脂酰肌醇甘露糖苷、一种未知的糖脂和一种未知的磷脂。主要的menaquinone 是 MK-8(H(4))。主要脂肪酸是 anteiso-C(15:0) 和 anteiso-C(15:1)。YIM 93316(T) 菌株的 DNA G+C 含量为 70.1mol%。化学分类学特性支持 YIM 93316(T) 菌株属于 Georgenia 属，这一结论得到了基于 16S rRNA 基因序列的系统发育分析的支持。YIM 93316(T) 菌株与 Georgenia thermotolerans TT02-04(T)、Georgenia ruanii YIM 004(T)和 Georgenia muralis 1A-C(T)的 16S rRNA 基因序列相似度分别为 96.6%、96.5%和 96.3%。脂肪酸、生理生化试验数据表明，YIM 93316(T) 菌株与公认的 Georgenia 属成员有明显的表型差异。基于本多相研究的证据，YIM 93316(T) 菌株被认为代表了 Georgenia 属的一个新种，建议将其命名为嗜盐乔治娜菌（Georgenia halophila）。模式菌株为 YIM 93316(T)（=DSM 21365(T)=CCTCC AB 208144(T)）。
