Key Laboratory for Microbial Resources of the Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, PR China.
Xinjiang Institute of Microbiology, Xinjiang Academy of Agricultural Science, Urumqi, Xinjiang, 830091, PR China.
Int J Syst Evol Microbiol. 2010 Sep;60(Pt 9):2113-2119. doi: 10.1099/ijs.0.018440-0. Epub 2009 Oct 23.
A Gram-staining-positive, facultatively anaerobic, non-motile and moderately halophilic actinobacterium, designated YIM 93306(T), was isolated from a salt lake in Xinjiang province, north-west China, and subjected to a polyphasic taxonomic study. Strain YIM 93306(T) grew in the presence of 2-16 % (w/v) NaCl and did not grow without NaCl. The peptidoglycan type was A4alpha with an l-Lys-l-Glu interpeptide bridge. The whole-cell sugars were glucosamine, arabinose, mannose and two unknown sugars. The predominant menaquinone was MK-8(H(4)). The major fatty acids were iso-C(15 : 0), anteiso-C(15 : 0) and anteiso-C(17 : 0). The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, one unknown phosphoglycolipid and one unknown phospholipid. The DNA G+C content was 68.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM 93306(T) fell within the radius of the suborder Micrococcineae. Its closest phylogenetic neighbour was the type strain of Ruania albidiflava (AS 4.3142(T); 96.2 % 16S rRNA gene sequence similarity), the sole recognized species of the genus Ruania. Sequence similarities between strain YIM 93306(T) and members of other genera of the suborder Micrococcineae were <95.2 %. On the basis of phylogenetic analysis, phenotypic characteristics and chemotaxonomic differences, a novel genus and species, Haloactinobacterium album gen. nov., sp. nov., is proposed. The type strain of the species is YIM 93306(T) (=DSM 21368(T) =KCTC 19413(T) =CCTCC AB 208069(T)). Based on phylogenetic characteristics and 16S rRNA gene signature nucleotide patterns, the genera Ruania and Haloactinobacterium gen. nov. are proposed to belong to a novel family, Ruaniaceae fam. nov.
一株革兰氏阳性、兼性厌氧、非运动和中度嗜盐放线菌，被命名为 YIM 93306(T)，从中国西北部新疆的一个盐湖中分离出来，并进行了多相分类学研究。菌株 YIM 93306(T)在 2-16%（w/v）NaCl 存在下生长，没有 NaCl 则不生长。肽聚糖类型为 A4alpha，具有 l-Lys-l-Glu interpeptide 桥。全细胞糖为葡萄糖胺、阿拉伯糖、甘露糖和两种未知糖。主要的类异戊二烯醌为 MK-8(H(4))。主要脂肪酸为 iso-C(15:0)、anteiso-C(15:0)和 anteiso-C(17:0)。极性脂包括双磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇、一种未知的磷酸甘油糖脂和一种未知的磷脂。DNA G+C 含量为 68.3mol%。基于 16S rRNA 基因序列的系统发育分析表明，菌株 YIM 93306(T)属于微球菌亚目。其最接近的系统发育近邻是 Ruania albidiflava (AS 4.3142(T))的模式株，这是 Ruania 属唯一被认可的物种。菌株 YIM 93306(T)与微球菌亚目中其他属的成员之间的序列相似性<95.2%。基于系统发育分析、表型特征和化学分类差异，提出了一个新属和新种，即 Haloactinobacterium album gen. nov.，sp. nov.。该种的模式株为 YIM 93306(T)=DSM 21368(T)=KCTC 19413(T)=CCTCC AB 208069(T)。根据系统发育特征和 16S rRNA 基因特征核苷酸模式，提出了 Ruania 和 Haloactinobacterium 属属于一个新的科，即 Ruaniaceae fam. nov.。
