Li Wen-Jun, Zhang Yu-Qin, Schumann Peter, Liu Hong-Yu, Yu Li-Yan, Zhang Yue-Qin, Stackebrandt Erko, Xu Li-Hua, Jiang Cheng-Lin
The Key Laboratory for Microbial Resources of the Ministry of Education, PR China.
Int J Syst Evol Microbiol. 2008 Jun;58(Pt 6):1359-63. doi: 10.1099/ijs.0.64226-0.
A Gram-positive, non-motile, moderately halophilic, alkalitolerant actinobacterium, designated strain YIM 70179(T), was isolated from a saline soil sample collected from Xinjiang Province, north-west China, and was subjected to a polyphasic taxonomic study. The cell-wall peptidoglycan type of strain YIM 70179(T) was A4alpha, l-Lys-Gly-l-Glu. Cells of the isolate contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unknown glycolipid, MK-8 as major menaquinone and anteiso-C(15 : 0), anteiso-C(17 : 0) and iso-C(16 : 0) as major fatty acids. The DNA G+C content was 68.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 70179(T) fell within the radiation of species of the genus Nesterenkonia. Levels of 16S rRNA gene sequence similarity between strain YIM 70179(T) and the type strains of recognized Nesterenkonia species were below 97 %, except to Nesterenkonia halobia DSM 20541(T) (99.6 %), but these two strains exhibited a low level of DNA-DNA relatedness (18.4 %). Based on genetic and phenotypic evidence, it is proposed that strain YIM 70179(T) represents a novel species of the genus Nesterenkonia, for which the name Nesterenkonia halophila sp. nov. is proposed. The type strain is YIM 70179(T) (=DSM 16378(T) =KCTC 19048(T)).
从中国西北部新疆采集的一份盐土样品中分离出一株革兰氏阳性、无运动性、中度嗜盐、耐碱的放线菌，命名为菌株YIM 70179(T)，并对其进行了多相分类研究。菌株YIM 70179(T)的细胞壁肽聚糖类型为A4α，l-Lys-Gly-l-Glu。该分离株的细胞含有双磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇和一种未知糖脂，MK-8为主要甲基萘醌，anteiso-C(15 : 0)、anteiso-C(17 : 0)和iso-C(16 : 0)为主要脂肪酸。DNA G+C含量为68.4 mol%。基于16S rRNA基因序列的系统发育分析表明，菌株YIM 70179(T)属于涅斯捷连科氏菌属物种的辐射范围。除了与嗜盐涅斯捷连科氏菌DSM 20541(T)（99.6%）外，菌株YIM 70179(T)与已确认的涅斯捷连科氏菌属物种的模式菌株之间的16S rRNA基因序列相似性水平低于97%，但这两个菌株的DNA-DNA相关性水平较低（18.4%）。基于遗传和表型证据，建议菌株YIM 70179(T)代表涅斯捷连科氏菌属的一个新物种，为此提出新名称嗜盐涅斯捷连科氏菌（Nesterenkonia halophila sp. nov.）。模式菌株为YIM 原文
A Gram-positive, non-motile, moderately halophilic, alkalitolerant actinobacterium, designated strain YIM 70179(T), was isolated from a saline soil sample collected from Xinjiang Province, north-west China, and was subjected to a polyphasic taxonomic study. The cell-wall peptidoglycan type of strain YIM 70179(T) was A4alpha, l-Lys-Gly-l-Glu. Cells of the isolate contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unknown glycolipid, MK-8 as major menaquinone and anteiso-C(15 : 0), anteiso-C(17 : 0) and iso-C(16 : 0) as major fatty acids. The DNA G+C content was 68.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 70179(T) fell within the radiation of species of the genus Nesterenkonia. Levels of 16S rRNA gene sequence similarity between strain YIM 70179(T) and the type strains of recognized Nesterenkonia species were below 97 %, except to Nesterenkonia halobia DSM 20541(T) (99.6 %), but these two strains exhibited a low level of DNA-DNA relatedness (18.4 %). Based on genetic and phenotypic evidence, it is proposed that strain YIM 70179(T) represents a novel species of the genus Nesterenkonia, for which the name Nesterenkonia halophila sp. nov. is proposed. The type strain is YIM 70179(T) (=DSM 16378(T) =KCTC 19048(T)).
从中国西北部新疆采集的一份盐土样品中分离出一株革兰氏阳性、无运动性、中度嗜盐、耐碱的放线菌，命名为菌株YIM 70179(T)，并对其进行了多相分类研究。菌株YIM 70179(T)的细胞壁肽聚糖类型为A4α，l-Lys-Gly-l-Glu。该分离株的细胞含有双磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇和一种未知糖脂，MK-8为主要甲基萘醌，anteiso-C(15 : ０)、anteiso-C(17 : ０)和iso-C(16 : ０)为主要脂肪酸。DNA G+C含量为68.4 mol%。基于16S rRNA基因序列的系统发育分析表明，菌株YIM 70179(T)属于涅斯捷连科氏菌属物种的辐射范围。除了与嗜盐涅斯捷连科氏菌DSM 20541(T)（99.6%）外，菌株YIM 70179(T)与已确认的涅斯捷连科氏菌属物种的模式菌株之间的16S rRNA基因序列相似性水平低于97%，但这两个菌株的DNA-DNA相关性水平较低（18.4%）。基于遗传和表型证据，建议菌株YIM 70179(T)代表涅斯捷连科氏菌属的一个新物种，为此提出新名称嗜盐涅斯捷连科氏菌（Nesterenkonia halophila sp. nov.）。模式菌株为YIM 70179(T)（=DSM 16378(T) =KCTC 19048(T)）。
