Kovárová H, Macela A, Stulík J
Department of Biochemistry, Hradec Králové.
Sb Ved Pr Lek Fak Karlovy Univerzity Hradci Kralove. 1990;33(5):565-77.
The activation of peritoneal macrophages in the course of primary infection of mice with attenuated strain of Francisella tularensis is associated with 2.5 fold increase in spontaneous INT reductase activity on day 5 after the immunization. The splenic cells of immunized mice pulsed in vitro by specific antigen secrete lymphokine that is able to induce an increase in spontaneous INT reductase activity of resident peritoneal cells. The production of spontaneous superoxide anion by peritoneal phagocytes reaches the highest level on day 5 after the immunization. It does not correlate with the results of cytotoxic or phagocytic activities at this time interval. An enhanced superoxide dismutase activity precedes an increase of superoxide anion secretion. The production of hydrogen peroxide is rising till day 7 and is related to the cytotoxic activity of peritoneal phagocytes. Concerning the testing of F. tularensis antigen as immunization agent, no changes of oxidative metabolism were detected. This might be in connection with the insufficient protection effect of killed F. tularensis vaccine. The production of reactive oxygen metabolites, probably under the control of superoxide dismutase, together with secreted lymphokines during the first days after the infection may play a regulatory role in the induction of immune response against intracellular pathogen F. tularensis.
用土拉弗朗西斯菌减毒株对小鼠进行初次感染时，腹膜巨噬细胞的激活与免疫后第5天自发INT还原酶活性增加2.5倍有关。经特异性抗原体外刺激的免疫小鼠脾细胞分泌的淋巴因子，能够诱导驻留腹膜细胞的自发INT还原酶活性增加。腹膜吞噬细胞自发产生超氧阴离子在免疫后第5天达到最高水平。在此时间间隔内，它与细胞毒性或吞噬活性的结果无关。超氧化物歧化酶活性增强先于超氧阴离子分泌增加。过氧化氢的产生一直上升到第7天，并且与腹膜吞噬细胞的细胞毒性活性有关。关于将土拉弗朗西斯菌抗原作为免疫剂进行检测，未检测到氧化代谢的变化。这可能与灭活的土拉弗朗西斯菌疫苗的保护作用不足有关。感染后的头几天，活性氧代谢产物的产生可能在超氧化物歧化酶的控制下，与分泌的淋巴因子一起，在针对细胞内病原体土拉弗朗西斯菌的免疫反应诱导中发挥调节作用。
