Kovárová H, Macela A, Stulík J
Department of Biochemistry, Faculty of Medicine, Charles University, Hradec Králové, Czechoslovakia.
Folia Microbiol (Praha). 1990;35(5):413-22. doi: 10.1007/BF02821410.
The changes of oxidative metabolism were studied in the course of a primary infection of mice with attenuated strain of Francisella tularensis. Metabolic stimulation of peritoneal cells is associated with a significant increase in spontaneous tetrazolium derivative reduction, the production of superoxide anion and hydrogen peroxide on day 5 after the immunization. The enhancement of superoxide dismutase precedes the increase in superoxide anion secretion. The splenic cells of immunized mice obtained on day 3 and in vitro pulsed by tularemic antigen secreted lymphokins(s) that could induce a metabolic stimulation. The treatment of resting splenic cells with hydrogen peroxide induces the secretion of interferon activity. The changes of oxidative metabolism that appear early after the infection seem to be related to a sequential activation of cells and probably have a regulatory role in the induction of immune defence against F. tularensis.
研究了用土拉弗朗西斯菌减毒株对小鼠进行初次感染过程中氧化代谢的变化。免疫后第5天，腹膜细胞的代谢刺激与自发四氮唑衍生物还原、超氧阴离子和过氧化氢生成的显著增加有关。超氧化物歧化酶的增强先于超氧阴离子分泌的增加。在第3天获得的免疫小鼠脾细胞，经土拉热抗原体外脉冲后可分泌能诱导代谢刺激的淋巴因子。用过氧化氢处理静止的脾细胞可诱导干扰素活性的分泌。感染后早期出现的氧化代谢变化似乎与细胞的顺序激活有关，并且可能在诱导针对土拉弗朗西斯菌的免疫防御中起调节作用。
