Bowei Chen, Xingyu Liu, Wenyan Liu, Jiankang Wen
National Engineering Laboratory of Biohydrometallurgy, General Research Institute for Nonferrous Metals, Beijing, China.
J Ind Microbiol Biotechnol. 2009 Nov;36(11):1409-16. doi: 10.1007/s10295-009-0627-7. Epub 2009 Aug 8.
The microbial communities of leachate from a bioleaching heap located in China were analyzed using the 16S rRNA gene clone library and real-time quantitative PCR. Both methods showed that Leptospirillum spp. were the dominant bacteria, and Ferroplasma acidiphilum were the only archaea detected in the leachate. Clone library results indicated that nine operational taxonomic units (OTUs) were obtained, which fell into four divisions, the Nitrospirae (74%), the gamma-Proteobacteria (14%), the Actinobacteria (6%) and the Euryarchaeota (6%). The results obtained by real-time PCR in some ways were the same as clone library analysis. Furthermore, Sulfobacillus spp., detected only by real-time PCR, suggests that real-time PCR was a reliable technology to study the microbial communities in bioleaching environments. It is a useful tool to assist clone library analysis, to further understand microbial consortia and to have comprehensive and exact microbiological information about bioleaching environments. Finally, the interactions among the microorganisms detected in the leachate were summarized according to the characteristics of these species.
采用16S rRNA基因克隆文库和实时定量PCR技术，对中国某生物沥浸堆渗滤液中的微生物群落进行了分析。两种方法均表明，钩端螺旋菌属是优势细菌，嗜酸亚铁原体是在渗滤液中检测到的唯一古菌。克隆文库结果表明，共获得9个操作分类单元（OTU），分属于4个门类，即硝化螺旋菌门（74%）、γ-变形菌纲（14%）、放线菌门（6%）和广古菌门（6%）。实时PCR获得的结果在某些方面与克隆文库分析结果相同。此外，仅通过实时PCR检测到的硫化杆菌属表明，实时PCR是研究生物沥浸环境中微生物群落的可靠技术。它是协助克隆文库分析、进一步了解微生物群落以及获取生物沥浸环境全面准确微生物信息的有用工具。最后，根据这些物种的特性总结了渗滤液中检测到的微生物之间的相互作用。
