The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, People's Republic of China.
J Ind Microbiol Biotechnol. 2012 Nov;39(11):1625-35. doi: 10.1007/s10295-012-1174-1. Epub 2012 Aug 8.
An extremely acidophilic sulfur-oxidizing bacterium was isolated from an industrial-scale bioheap of the Zijinshan copper mine and was named ZJJN. A tuft of flagella and a layer of thick capsule outside the cell envelope were clearly observed under transmission electron microscopy (TEM), which might be closely related to the extremely acid-proof capacity of ZJJN cells in the bioleaching system; 16S ribosomal RNA (rRNA) phylogeny showed that the isolated strain was highly homologous to the genera of Acidithiobacillus. The optimum temperature of ZJJN was determined at 30 °C and pH at 1.0. It was capable of growth at even pH 0. Strain ZJJN can utilize reduced sulfur as an energy source but not with organics or ferrous ion. Strain ZJJN was sensitive to all antibiotics with different concentrations; when it showed a certain resistance to different concentrations of Cu(2+). In the mixed strains of ZJJN and A. ferrooxidans system (initial pH 1.0), the copper-leaching efficiency was up to 60.1 %, which was far higher than other systems. Scanning electron microscopy (SEM) analysis showed that less jarosite precipitation was produced in the most efficient system. The extremely acidophilic strain ZJJN would be of great potential in the application of chalcopyrite bioleaching.
从工业规模的紫金山铜矿生物堆中分离到一株极端嗜酸硫氧化菌，并将其命名为 ZJJN。透射电子显微镜（TEM）下清晰观察到细胞包膜外有一束鞭毛和一层厚厚的荚膜，这可能与 ZJJN 细胞在生物浸出系统中极强的耐酸能力密切相关；16S 核糖体 RNA（rRNA）系统发育分析表明，分离得到的菌株与嗜酸硫杆菌属高度同源。ZJJN 的最适温度为 30°C，最适 pH 为 1.0。它甚至可以在 pH 值为 0 的条件下生长。菌株 ZJJN 可以利用还原态硫作为能源，但不能利用有机物或亚铁离子。菌株 ZJJN 对不同浓度的所有抗生素都很敏感；当它对不同浓度的 Cu(2+)表现出一定的抗性时。在 ZJJN 和 A. ferrooxidans 混合菌株系统（初始 pH 1.0）中，铜浸出效率高达 60.1%，远高于其他系统。扫描电子显微镜（SEM）分析表明，在效率最高的系统中产生的铁矾沉淀较少。极端嗜酸菌株 ZJJN 在黄铜矿生物浸出中的应用具有很大的潜力。
