el-Hakim I E, Langdon J D, Zakrzewski J T, Costello J F
Department of Oral and Maxillofacial Surgery, King's College School of Medicine and Dentistry, Denmark Hill, London.
Br J Oral Maxillofac Surg. 1990 Jun;28(3):155-9. doi: 10.1016/0266-4356(90)90078-y.
The quantitation of leukotriene B4 (LTB4) in induced squamous cell carcinoma (SCC) of the Syrian hamster cheek pouch and histologically proven human oral SCC was investigated by a combination of reverse phase-high performance liquid chromatography (RP-HPLC) and radioimmunoassay (RIA). Healthy tissue obtained from these same patients and animals treated with vehicle alone were used as controls. From both animal and human studies our results show a 10 to 30 fold increase in the levels of LTB4 found in tumour compared to control tissue. Furthermore, this dihydroxy acid was not detected in the mucosal tissue of normal subjects undergoing routine surgery. Since LTB4 is a potent inflammatory mediator and modulator of immune responses, its presence at biologically active concentrations in human squamous cell carcinoma suggests a possible role in the pathogenesis of head and neck cancer.
采用反相高效液相色谱法（RP-HPLC）和放射免疫分析法（RIA）相结合的方法，对叙利亚仓鼠颊囊诱发性鳞状细胞癌（SCC）和组织学确诊的人类口腔SCC中白三烯B4（LTB4）进行定量分析。从这些相同的患者和仅用赋形剂治疗的动物身上获取的健康组织用作对照。动物和人体研究结果均显示，与对照组织相比，肿瘤中LTB4水平升高了10至30倍。此外，在接受常规手术的正常受试者的粘膜组织中未检测到这种二羟基酸。由于LTB4是一种强效的炎症介质和免疫反应调节剂，其在人类鳞状细胞癌中以生物活性浓度存在，提示其在头颈部癌发病机制中可能发挥作用。
