Melkikh Alexey V, Seleznev Vladimir D
Ural State Technical University, Yekaterinburg, Russia.
J Biol Phys. 2007 Apr;33(2):161-70. doi: 10.1007/s10867-007-9053-0. Epub 2008 Jan 22.
A model of the active transport of ions through internal membranes of mitochondria is proposed. If concentrations of ions in a cell are known, this model allows calculating concentrations of all main ions (H(+), Ca(+2), K(+), Mg(2+), Na(+), Cl(-)) in the mitochondrion matrix and the resting potential across the membrane. The theoretical values satisfactorily agree with available experimental data on the concentrations and the potentials, including different operating regimes of the adenosine triphosphate (ATP) synthetase (the main regime, short circuiting or ATP synthetase blocking). The active transport of Mg(2+) ions in exchange for protons was assumed. In accordance with the model, the ATP synthetase operation is possible only if the stoichiometric coefficient of protons is 3.
提出了一种离子通过线粒体内膜主动运输的模型。如果已知细胞中离子的浓度，该模型可以计算线粒体基质中所有主要离子（H⁺、Ca²⁺、K⁺、Mg²⁺、Na⁺、Cl⁻）的浓度以及跨膜静息电位。理论值与关于浓度和电位的现有实验数据令人满意地相符，包括三磷酸腺苷（ATP）合酶的不同运行模式（主要模式、短路或ATP合酶阻断）。假定Mg²⁺离子与质子进行交换的主动运输。根据该模型，只有当质子的化学计量系数为3时，ATP合酶才能运行。
