Stromberg P C, Grants I S, Reiter J A, Krakowka G S, Kociba G J, Mezza L E, Rinehart J J
Department of Veterinary Pathobiology, Ohio State University, Columbus 43210-1093.
J Clin Lab Immunol. 1990 Jan;31(1):27-31.
The effect of large granular lymphocyte leukemia on B lymphocyte function was studied by determining the number of plaques formed in an in vitro hemolytic plaque assay. Leukemia cells inhibited plaque formation by normal splenic lymphocytes in a logarithmic, dose-dependent manner. At the highest leukemia cell concentrations, spleen cell suspensions made 50% fewer plaques. Plaque forming responses were very sensitive to duration of preincubation time in all assays. The number of plaques formed decreased markedly if incubated 2 hr before the assay was performed. Incubation of the cells at 56 degrees C for 8 min did not alter the inhibitory activity but pretreatment with 0.01% trypsin did. Supernatant fluids from leukemia cell suspensions did not inhibit plaque formation. These data suggest that diffuse infiltration of lymphoid tissues by leukemia cells may interfere with some normal lymphocyte functions. Although leukemia cells inhibited splenic B lymphocyte function, leukemic rats did not have hypogammaglobulinemia.
通过在体外溶血空斑试验中测定形成的空斑数量，研究了大颗粒淋巴细胞白血病对B淋巴细胞功能的影响。白血病细胞以对数、剂量依赖性方式抑制正常脾淋巴细胞形成空斑。在最高白血病细胞浓度下，脾细胞悬液形成的空斑减少50%。在所有试验中，空斑形成反应对预孵育时间的长短非常敏感。如果在进行试验前孵育2小时，形成的空斑数量会显著减少。将细胞在56℃孵育8分钟不会改变抑制活性，但用0.01%胰蛋白酶预处理则会改变。白血病细胞悬液的上清液不抑制空斑形成。这些数据表明，白血病细胞对淋巴组织的弥漫性浸润可能会干扰某些正常淋巴细胞功能。虽然白血病细胞抑制了脾脏B淋巴细胞功能，但白血病大鼠并没有低丙种球蛋白血症。
