Stromberg P C, Vogtsberger L M, McMurray D N, Marsh L R, Kotur M S, Brown C A
Lab Invest. 1985 Aug;53(2):200-8.
The behavior of transplanted large granular lymphocyte leukemia was studied by isogeneic inoculation of 55 young F344 rats. A high percentage of transplants were successful, showing a dose-dependent latent phase followed by weight loss, exponential increase of tumor cells, and widespread infiltration. Serial passage of tumor cells appeared to increase malignancy by decreasing longevity. Grossly, there was splenomegaly, variable lymphadenopathy, and petechial hemorrhages on the lungs, lymph nodes, and brain. Diffuse infiltration of tumor cells caused morphologic changes identical with spontaneous large granular lymphocyte leukemia including splenic lymphoid depletion, hepatocellular necrosis, tumor cell erythrophagocytosis, and femoral medullary endosteal bone proliferation. Clinicopathologic features were also similar to spontaneous large granular lymphocyte leukemia. There was immune-mediated hemolytic anemia and thrombocytopenia, which coincided with development of leukemia, as well as hyperbilirubinemia and elevated serum enzymes, indicative of liver disease. It was concluded that isogeneic transplantation of large granular lymphocyte leukemia is not only highly successful but reproduces the pattern and clinical syndrome of the spontaneous tumor including the important paraneoplastic syndrome of hemolytic anemia and thrombocytopenia.
通过对55只年轻的F344大鼠进行同基因接种，研究了移植性大颗粒淋巴细胞白血病的行为。移植成功率很高，呈现出剂量依赖性的潜伏期，随后出现体重减轻、肿瘤细胞指数性增加以及广泛浸润。肿瘤细胞的连续传代似乎通过缩短生存期来增加恶性程度。大体上，有脾肿大、不同程度的淋巴结病以及肺、淋巴结和脑的瘀点性出血。肿瘤细胞的弥漫性浸润导致了与自发性大颗粒淋巴细胞白血病相同的形态学变化，包括脾淋巴细胞耗竭、肝细胞坏死、肿瘤细胞吞噬红细胞以及股骨骨髓内骨增生。临床病理特征也与自发性大颗粒淋巴细胞白血病相似。存在免疫介导的溶血性贫血和血小板减少症，这与白血病的发展同时出现，还有高胆红素血症和血清酶升高，提示肝脏疾病。结论是，大颗粒淋巴细胞白血病的同基因移植不仅非常成功，而且重现了自发性肿瘤的模式和临床综合征，包括溶血性贫血和血小板减少症这一重要的副肿瘤综合征。
