State Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Nanjing Agricultural University, Nanjing, 210095, China.
Mol Biotechnol. 2010 Jan;44(1):30-40. doi: 10.1007/s12033-009-9202-8. Epub 2009 Aug 11.
The plant-specific NAC (for NAM, ATAF1,2 and CUC2) proteins have been found to play important roles in plant development and stress responses. In this study, a NAC gene CarNAC1 (for Cicer arietinum L. NAC gene 1) was isolated from a cDNA library constructed with chickpea seedling leaves treated by polyethylene glycol. CarNAC1 encoded a putative protein with 239 amino acids and contained 3 exons and 2 introns within genomic DNA sequence. CarNAC1 had a conserved NAC domain in the N-terminus and the CarNAC1:GFP (green fluorescent protein) fusion protein was localized in the nucleus of onion epidermal cells. Additionally, CarNAC1 exhibited the trans-activation activity which was mapped to the C-terminus. The CarNAC1 transcript was detected in many chickpea organs including seedling leaves, stems, roots, flowers, and young pods, but less accumulated in young seeds. CarNAC1 was induced by leaf age and showed changes in expression during seed development and germination. Furthermore, the expression of CarNAC1 was strongly induced by drought, salt, cold, wounding, H(2)O(2), ethephon, salicylic acid, indole-3-acetic acid, and gibberellin. Our results suggest that CarNAC1 encodes a novel NAC-domain protein and may be a transcriptional activator involved in plant development and various stress responses.
植物特异性 NAC（NAM、ATAF1、2 和 CUC2）蛋白被发现在植物发育和应激反应中发挥重要作用。本研究从用聚乙二醇处理的鹰嘴豆幼苗叶片构建的 cDNA 文库中分离出一个 NAC 基因 CarNAC1（Cicer arietinum L. NAC 基因 1）。CarNAC1 编码一个推定的蛋白，含有 239 个氨基酸，在基因组 DNA 序列中包含 3 个外显子和 2 个内含子。CarNAC1 在 N 端具有保守的 NAC 结构域，CarNAC1:GFP（绿色荧光蛋白）融合蛋白定位于洋葱表皮细胞的细胞核中。此外，CarNAC1 表现出跨激活活性，该活性被定位在 C 端。CarNAC1 转录本在鹰嘴豆的许多器官中都有检测到，包括幼苗叶片、茎、根、花和幼荚，但在幼种子中积累较少。CarNAC1 受叶片年龄诱导，并在种子发育和萌发过程中表达发生变化。此外，CarNAC1 的表达受干旱、盐、冷、创伤、H2O2、乙烯、水杨酸、吲哚-3-乙酸和赤霉素强烈诱导。我们的结果表明，CarNAC1 编码一种新型的 NAC 结构域蛋白，可能是一种参与植物发育和各种应激反应的转录激活因子。
