Li Jing, Cai Tanxi, Wu Peng, Cui Ziyou, Chen Xiulan, Hou Junjie, Xie Zhensheng, Xue Peng, Shi Linan, Liu Pingsheng, Yates John R, Yang Fuquan
Proteomic Platform, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, PR China.
Proteomics. 2009 Oct;9(19):4539-53. doi: 10.1002/pmic.200900101.
Mitochondria play essential roles in cell physiological processes including energy production, metabolism, ion homeostasis, cell growth, aging and apoptosis. Proteomic strategies have been applied to the study of mitochondria since 1998; these studies have yielded decisive information about the diverse physiological functions of the organelle. As an ideal model biological system, the nematode Caenorhabditis elegans has been widely used in the study of several diseases, such as metabolic diseases and cancer. However, the mitochondrial proteome of C. elegans remains elusive. In this study, we purified mitochondria from C. elegans and performed a comprehensive proteomic analysis using the shotgun proteomic approach. A total of 1117 proteins have been identified with at least two unique peptides. Their physicochemical and functional characteristics, subcellular locations, related biological processes, and associations with human diseases, especially Parkinson's disease, are discussed. An orthology comparison was also performed between C. elegans and four other model organisms for a general depiction of the conservation of mitochondrial proteins during evolution. This study will provide new clues for understanding the role of mitochondria in the physiological and pathological processes of C. elegans.
线粒体在细胞生理过程中发挥着重要作用，包括能量产生、新陈代谢、离子稳态、细胞生长、衰老和凋亡。自1998年以来，蛋白质组学策略已应用于线粒体研究；这些研究已经产生了关于该细胞器多种生理功能的决定性信息。作为一种理想的模式生物系统，线虫秀丽隐杆线虫已被广泛用于多种疾病的研究，如代谢疾病和癌症。然而，秀丽隐杆线虫的线粒体蛋白质组仍然难以捉摸。在本研究中，我们从秀丽隐杆线虫中纯化出线粒体，并使用鸟枪法蛋白质组学方法进行了全面的蛋白质组分析。共鉴定出1117种蛋白质，每种蛋白质至少有两个独特的肽段。讨论了它们的理化和功能特性、亚细胞定位、相关生物学过程以及与人类疾病尤其是帕金森病的关联。还对线虫与其他四种模式生物进行了直系同源比较，以总体描述线粒体蛋白质在进化过程中的保守性。本研究将为理解线粒体在秀丽隐杆线虫生理和病理过程中的作用提供新的线索。
