[单侧迷路切除术后大鼠内侧前庭核中代谢型谷氨酸受体5的表达变化]
[Expression change of mGluR5 in rat MVN after unilateral labyrinthectomy].
作者信息
Zhang Yamin, Kong Weijia, Hu Yujuan
机构信息
Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
出版信息
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2009 May;23(10):456-9.
OBJECTIVE
To observe the expression of mGluR5 in the medial vestibular nucleus (MVN) following unilateral labyrinthectomy (UL).
METHOD
Thirty Wistar rats were randomly divided into two groups. Twenty four animals received unilateral labyrinthectomy while the others maintained labyrinthine well. After setting left labyrinthine, the change of mGluR5 was induced by immunohistochemistry, in situ hybridization.
RESULT
mGluR5 was increased in lesioned side MVN after unilateral labyrinthectomy. The 12 h post-UL was highest. Then it was decrease in 36 h post-UL, while 7 d post-UL was same as control group. The change of contralateral was same as that in ipsilateral.
CONCLUSION
UL can induce increase of mGluR5 in the MVN. The reduced resting discharge in the primary vestibular afferents or in the central vestibular neurons may be responsible for the change of mGluR5. However the significance of the change of mGluR1 in the vestibular compensation is still unknown.
目的
观察单侧迷路切除术后内侧前庭核（MVN）中代谢型谷氨酸受体5（mGluR5）的表达。
方法
将30只Wistar大鼠随机分为两组。24只动物接受单侧迷路切除术，其余动物保持迷路完整。在设定左侧迷路后，通过免疫组织化学、原位杂交诱导mGluR5的变化。
结果
单侧迷路切除术后，损伤侧MVN中mGluR5增加。术后12小时最高。然后在术后36小时下降，而术后7天与对照组相同。对侧的变化与同侧相同。
结论
单侧迷路切除术可诱导MVN中mGluR5增加。初级前庭传入纤维或中枢前庭神经元静息放电减少可能是mGluR5变化的原因。然而，mGluR1变化在前庭代偿中的意义仍不清楚。
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