Silver Annemarie E, Christou Demetra D, Donato Anthony J, Beske Stacy D, Moreau Kerrie L, Magerko Katherine A, Seals Douglas R
Department of Integrative Physiology, University of Colorado, Boulder, CO 80309,USA.
J Vasc Res. 2010;47(1):1-8. doi: 10.1159/000231715. Epub 2009 Aug 6.
Studying molecular mechanisms of vascular endothelial function in humans is difficult in part because of limited access to arteries. Access to peripheral veins is more practical. We determined if differences in protein expression of endothelial cells (EC) collected from a peripheral artery are reflected in measurements made on EC obtained from peripheral veins. EC were collected from the brachial artery and an antecubital vein of 106 healthy adults (60 men and 46 women, age 18-77 years). Quantitative immunofluorescence was used to measure protein expression of endothelial nitric oxide synthase (eNOS), Ser-1177 phosphorylated eNOS, manganese superoxide dismutase, nitrotyrosine, xanthine oxidase and nuclear factor-kappaB p65. Protein expression in EC obtained from brachial artery and antecubital vein sampling was moderately to strongly related (r = 0.59-0.81, all p < 0.0001, mean r = 0.70). Moreover, differences between subgroups in the lowest and highest tertiles of protein expression in EC obtained from arterial samples were consistently reflected in EC obtained from venous collections. These findings indicate that interindividual and group differences in expression of several proteins involved in nitric oxide production, oxidant production, antioxidant defense and inflammatory signaling in EC obtained from brachial artery sampling are consistently reflected in EC obtained from venous samples. Thus, EC collected from peripheral veins may provide a useful surrogate for EC obtained from arteries for measurements of EC protein expression in humans.
研究人类血管内皮功能的分子机制存在困难，部分原因是获取动脉的机会有限。获取外周静脉则更为可行。我们确定了从外周动脉采集的内皮细胞（EC）的蛋白质表达差异是否反映在从外周静脉获取的EC的测量结果中。从106名健康成年人（60名男性和46名女性，年龄18 - 77岁）的肱动脉和肘前静脉采集EC。采用定量免疫荧光法测量内皮型一氧化氮合酶（eNOS）、丝氨酸1177磷酸化eNOS、锰超氧化物歧化酶、硝基酪氨酸、黄嘌呤氧化酶和核因子-κB p65的蛋白质表达。从肱动脉和肘前静脉采样获得的EC中的蛋白质表达呈中度至强相关（r = 0.59 - 0.81，所有p < 0.0001，平均r = 0.70）。此外，动脉样本中EC蛋白质表达最低和最高三分位数亚组之间的差异始终反映在静脉采集的EC中。这些发现表明，从肱动脉采样获得的EC中参与一氧化氮产生、氧化剂产生、抗氧化防御和炎症信号传导的几种蛋白质表达的个体间和组间差异始终反映在静脉样本的EC中。因此，从外周静脉采集的EC可能为从动脉获取的EC提供一个有用的替代物，用于测量人类EC的蛋白质表达。
