Mann D L, Gartner S, LeSane F, Blattner W A, Popovic M
Immunogenetics Section, National Cancer Institute, Frederick Cancer Research Facility, Maryland 21701.
Clin Immunol Immunopathol. 1990 Feb;54(2):174-83. doi: 10.1016/0090-1229(90)90079-6.
The human immunodeficiency virus (HIV-1) preferentially infects cells that express the CD4 molecule, including monocytes and cells of the monocyte lineage. The monocyte-like cell line U937 and monocytes isolated from peripheral blood lymphocytes (PBL) were infected with HIV-1. Cell surface antigen expression was determined in infected and noninfected cells as was the ability to stimulate in mixed lymphocyte reaction. The CD4 antigen decreased in infected cells U937 and PBL monocytes. MHC class II antigens HLA-DR, HLA-DQ, and HLA-DP increased in HIV-1 infected U937 cells. In infected PBL-derived monocytes, HLA-DR increased, HLA-DQ decreased, and HLA-DP was unchanged. Infected U937 and PBL monocytes were capable of stimulating allogeneic lymphocytes, thus demonstrating retention of the alloantigen presentation function of HIV-1-infected monocytes.
人类免疫缺陷病毒（HIV-1）优先感染表达CD4分子的细胞，包括单核细胞和单核细胞系的细胞。用HIV-1感染单核细胞样细胞系U937和从外周血淋巴细胞（PBL）中分离出的单核细胞。测定感染和未感染细胞的细胞表面抗原表达以及混合淋巴细胞反应中的刺激能力。在感染的U937细胞和PBL单核细胞中，CD4抗原减少。在HIV-1感染的U937细胞中，MHC II类抗原HLA-DR、HLA-DQ和HLA-DP增加。在感染的PBL来源的单核细胞中，HLA-DR增加，HLA-DQ减少，HLA-DP不变。感染的U937和PBL单核细胞能够刺激同种异体淋巴细胞，从而证明HIV-1感染的单核细胞保留了同种抗原呈递功能。
