Melendez-Guerrero L M, Nicholson J K, McDougal J S
Department of Pathology, Emory University School of Medicine, Atlanta, Georgia 30322.
AIDS Res Hum Retroviruses. 1990 Jun;6(6):731-41. doi: 10.1089/aid.1990.6.731.
Monocytes from healthy blood donors were inoculated in vitro with a monocyte-tropic strain of human immunodeficiency virus type 1 HIVBa-L. HIV replication was first detected at Day 5 postinoculation, with peak virus activity at Day 17. We assessed the kinetics of the expression of four monocyte surface antigens (CD14, CD4, HLA-DR, and HLA-DQ) on HIV-infected and uninfected monocyte/macrophages, (M phi) by flow cytometry. We consistently found a decreased expression of CD4 and CD14 on HIV-infected M phi compared to their expression on M phi of uninfected controls. In contrast, HLA-DR and HLA-DQ expression was unchanged on HIV-infected M phi.
将来自健康献血者的单核细胞在体外接种1型人类免疫缺陷病毒（HIV）的嗜单核细胞株HIVBa-L。接种后第5天首次检测到HIV复制，第17天病毒活性达到峰值。我们通过流式细胞术评估了HIV感染和未感染的单核细胞/巨噬细胞（M phi）上四种单核细胞表面抗原（CD14、CD4、HLA-DR和HLA-DQ）的表达动力学。我们始终发现，与未感染对照的M phi相比，HIV感染的M phi上CD4和CD14的表达降低。相比之下，HIV感染的M phi上HLA-DR和HLA-DQ的表达没有变化。
