Shi Yan-Ting, Si Chun-Feng, Liu Bin
The Second Affiliated Hospital of Shandong University, Jinan.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2009 May;29(5):422-4.
To study the kinetogenic effects of serum containing Semen Arecae, Dandelion, Semen raphani and Atractylodes macrocephala on the colonic smooth muscle cells of rats.
Serum containing Chinese materia medicas was made according to standard methods. Smooth muscle cells were isolated from the muscle layers of Wistar rat's colon, referred to modified Bitar's method. The contractile response of colonic smooth muscle cells to serum containing Chinese materia medicas (10%, 50%, 100% concentration) and other medicines (blank and 1 x 10(-3) mol/L acetylcholine) were separately observed. The contractility was presented by the decrease of the cell length between the drug groups and the control.
Serum containing each Chinese materia medica can make dose-dependent contraction at different concentrations (P < 0.05), but the strongest effect of each serum had no significant difference (P > 0.05).
Serum containing Semen Arecae, Dandelion, Semen raphani and Atractylodes macrocephala can make notable contraction on colonic smooth muscle cells in rats.
研究含槟榔、蒲公英、莱菔子和白术血清对大鼠结肠平滑肌细胞的促动效应。
按照标准方法制备含中药血清。采用改良的比塔尔方法从Wistar大鼠结肠肌层分离平滑肌细胞。分别观察结肠平滑肌细胞对含中药血清（浓度为10%、50%、100%）和其他药物（空白及1×10⁻³mol/L乙酰胆碱）的收缩反应。以药物组与对照组之间细胞长度的减少来表示收缩力。
含各味中药的血清在不同浓度下均可产生剂量依赖性收缩（P<0.05），但各血清的最强效应无显著差异（P>0.05）。
含槟榔、蒲公英、莱菔子和白术的血清可使大鼠结肠平滑肌细胞产生显著收缩。
