氟化脂质构建体可使分子货物轻松进入活细胞。
Fluorinated lipid constructs permit facile passage of molecular cargo into living cells.
作者信息
Dafik Laila, Kalsani Venkateshwarlu, Leung Anthony Kar Lun, Kumar Krishna
机构信息
Department of Chemistry, Tufts University, Medford, Massachusetts 02155, USA.
出版信息
J Am Chem Soc. 2009 Sep 2;131(34):12091-3. doi: 10.1021/ja902777d.
Abstract
Fluorinated lipids get rapidly internalized into living cells and are also displayed on the cell surface. The uptake of lipids is energy dependent and is likely via the clathrin-mediated endocytic pathway. Fluorinated lipids are 3-5-fold more efficient in acting as molecular transporters of noncovalently bound proteins than their hydrocarbon counterparts. These materials could serve as efficient molecular transporters for molecules that function in the cytoplasm such as short interfering RNAs (siRNAs).
摘要
氟化脂质能迅速被活细胞内化，并且也会展示在细胞表面。脂质的摄取依赖能量，可能通过网格蛋白介导的内吞途径进行。与相应的碳氢化合物相比，氟化脂质作为非共价结合蛋白的分子转运体的效率要高3至5倍。这些材料可作为细胞质中发挥作用的分子（如小干扰RNA（siRNA））的有效分子转运体。
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