Salvador Marcos J, Pereira Paulo S, França Suzelei C, Candido Regina C, Ito Izabel Y, Dias Diones A
Curso de Farmácia, Departamento de Biologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), C.P. 6109, 13083-970, Campinas, SP, Brazil.
Z Naturforsch C J Biosci. 2009 May-Jun;64(5-6):373-81. doi: 10.1515/znc-2009-5-612.
Crude extracts of a callus culture (two culture media) and adult plants (two collections) from Alternanthera tenella Colla (Amaranthaceae) were evaluated for their antibacterial and antifungal activity, in order to investigate the maintenance of antimicrobial activity of the extracts obtained from plants in vivo and in vitro. The antibacterial and antifungal activity was determined against thirty strains of microorganisms including Gram-positive and Gram-negative bacteria, yeasts and dermatophytes. Ethanolic and hexanic extracts of adult plants collected during the same period of the years 1997 and 2002 [Ribeirão Preto (SP), collections 1 and 2] and obtained from plant cell callus culture in two different hormonal media (AtT43 and AtT11) inhibited the growth of bacteria, yeasts and dermatophytes with inhibition halos between 6 and 20 mm. For the crude extracts of adult plants bioassay-guided fractionation, purification, and isolation were performed by chromatographic methods, and the structures of the isolated compounds were established by analysis of chemical and spectral evidences (UV, IR, NMR and ES-MS). Steroids, saponins and flavonoids (aglycones and C-glycosides) were isolated. The minimum inhibitory concentration (MIC) of the isolated compounds varied from 50 to 500 microg/mL.
对来自千日红（苋科）的愈伤组织培养物（两种培养基）和成年植株（两个采集地）的粗提物进行了抗菌和抗真菌活性评估，以研究从植物体内和体外获得的提取物的抗菌活性维持情况。针对包括革兰氏阳性菌、革兰氏阴性菌、酵母和皮肤癣菌在内的30种微生物菌株测定了抗菌和抗真菌活性。1997年和2002年同期采集于里贝朗普雷图（圣保罗）的成年植株（采集地1和2）的乙醇提取物和己烷提取物，以及在两种不同激素培养基（AtT43和AtT11）中获得的植物细胞愈伤组织培养物提取物，均能抑制细菌、酵母和皮肤癣菌的生长，抑菌圈直径在6至20毫米之间。对于成年植株的粗提物，通过色谱方法进行生物测定导向的分级分离、纯化和分离，并通过化学和光谱证据（紫外、红外、核磁共振和电喷雾质谱）分析确定分离化合物的结构。分离出了类固醇、皂苷和黄酮类化合物（苷元及碳苷）。分离化合物的最低抑菌浓度（MIC）在50至500微克/毫升之间。
