Mora Laura, Chumbimuni-Torres Karin Y, Clawson Corbin, Hernandez Lucas, Zhang Liangfang, Wang Joseph
Department of Nanoengineering, University of California San Diego, La Jolla, CA 92093, USA; Department of Analytical Chemistry, Universidad Autónoma de Madrid, 28049 Cantoblanco, Madrid, Spain.
J Control Release. 2009 Nov 16;140(1):69-73. doi: 10.1016/j.jconrel.2009.08.002. Epub 2009 Aug 11.
An electrochemical protocol for real-time monitoring of drug release kinetics from therapeutic nanoparticles (NPs) is described. The method is illustrated for repetitive square-wave voltammetric measurements of the reduction of doxorubicin released from liposomes at a glassy-carbon electrode. Such operation couples high sensitivity down to 20 nM doxorubicin with high speed and stability. It can thus monitor in real time the drug release from NP carriers, including continuous measurements in diluted serum. Such direct and continuous monitoring of the drug release kinetics from therapeutic NPs holds great promise for designing new drug delivery NPs with optimal drug release properties. These NPs can potentially be used to deliver many novel compounds such as marine-life derived drugs and hydrophobic drugs with limited water solubility that are usually difficult to be characterized by traditional analytical tools.
本文描述了一种用于实时监测治疗性纳米颗粒（NPs）药物释放动力学的电化学方法。该方法通过在玻碳电极上对从脂质体释放的阿霉素还原进行重复方波伏安测量来说明。这种操作将低至20 nM阿霉素的高灵敏度与高速和稳定性相结合。因此，它可以实时监测NP载体的药物释放，包括在稀释血清中的连续测量。这种对治疗性NPs药物释放动力学的直接和连续监测对于设计具有最佳药物释放特性的新型药物递送NPs具有很大的前景。这些NPs有可能用于递送许多新型化合物，如海洋生物衍生药物和水溶性有限的疏水性药物，而这些药物通常难以用传统分析工具进行表征。
