两种酸溶液和 2.5%次氯酸钠用于牙髓治疗的细胞毒性作用。
Cytotoxic effects of two acid solutions and 2.5% sodium hypochlorite used in endodontic therapy.
机构信息
Department of Dental Pathology and Therapeutics, School of Dentistry, University of Granada, Granada, Spain.
出版信息
Med Oral Patol Oral Cir Bucal. 2010 Jan 1;15(1):e90-4. doi: 10.4317/medoral.15.e90.
AIM
To evaluate the cytotoxicity of 15% citric acid, 5% phosphoric acid and 2.5% NaOCl on cultured fibroblasts using MTT colorimetric assay.
METHODOLOGY
Irrigating solutions of 5% phosphoric acid, 15% citric acid, and 2.5% NaOCl, diluted at 0.1% and 0.5%, were applied to cell cultures of 3T3L1 fibroblasts. The cell viability was determined by means of MTT colorimetric assay after a period of 1, 6 and 24 hours. Percentages of cell viability were analyzed using the Kruskal-Wallis test for global comparisons and the Mann-Whitney U-test for pairwise comparisons.
RESULTS
The percentage of cell viability diminished progressively over a 24 hour period in all solutions at both dilutions. At 0.1% dilution, 2.5% NaOCl (63.39%) and 15% citric acid (53.91%) showed the highest percentage of cell viability (p=0.083). At 0.5% dilution, 2.5% NaOCl again showed the highest cell viability value (48.51%).
CONCLUSIONS
The irrigating solution with the highest percentage of cell viability was 2.5% NaOCl at both 0.1% and 0.5% dilutions. A very low percentage of cell viability was obtained with 15% citric acid and 5% phosphoric acid at 0.5% dilution.
目的
使用 MTT 比色法评估 15%柠檬酸、5%磷酸和 2.5%次氯酸钠对培养成纤维细胞的细胞毒性。
方法
将 5%磷酸、15%柠檬酸和 2.5%次氯酸钠的冲洗液稀释至 0.1%和 0.5%，应用于 3T3L1 成纤维细胞的细胞培养物。用 MTT 比色法测定细胞活力，在 1、6 和 24 小时后测定。用 Kruskal-Wallis 检验进行全局比较，用 Mann-Whitney U 检验进行两两比较，分析细胞活力的百分比。
结果
在所有稀释度的溶液中，细胞活力在 24 小时内逐渐降低。在 0.1%的稀释度下，2.5%次氯酸钠（63.39%）和 15%柠檬酸（53.91%）显示出最高的细胞活力百分比（p=0.083）。在 0.5%的稀释度下，2.5%次氯酸钠再次显示出最高的细胞活力值（48.51%）。
结论
在 0.1%和 0.5%的稀释度下，细胞活力最高的冲洗液是 2.5%次氯酸钠。在 0.5%的稀释度下，15%柠檬酸和 5%磷酸的细胞活力百分比非常低。
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