Camacho-Alonso Fabio, Salmerón-Lozano P, Martínez-Beneyto Y
Clínica Odontológica Universitaria, Department of Oral Surgery, Hospital Morales Meseguer (2ª floor), University of Murcia, Avda. Marqués de los Vélez s/n, 30008, Murcia, Spain.
Clinical Teaching Fellow, University of Manchester, Manchester, UK.
Odontology. 2017 Jul;105(3):338-346. doi: 10.1007/s10266-016-0271-4. Epub 2016 Oct 22.
The aim of this study was to evaluate the antibacterial effect of photodynamic therapy (PDT), 2 % chlorhexidine (CHX). The teeth were contaminated with 0.1 mL Enterococcus faecalis (3 × 10 cell/mL) and randomized into eight treatment groups: Group 1 (2.5 % NaOCl); Group 2 (PDT); Group 3 (2 % CHX); Group 4 (TAM); Group 5 (propolis), Group 6 (ozone), Group 7 (positive control) and Group 8 (negative control). Following treatment, the canal content was sampled. The samples were cultured in triplicate on blood agar plates to determine the number of colony forming units (CFU)/mL. The teeth were analyzed by SEM to determine the percentage of area with contamination and debris. The group with the lowest CFU/mL count was the ozone group, which obtained similar values to the PDT group. SEM images showed that 2.5 % NaOCL group showed the lowest percentage of contaminated area. Applications of PDT, 2 % CHX, TAM, propolis and ozone showed antibacterial potential similar to 2.5 % NaOCL.
本研究的目的是评估光动力疗法（PDT）、2%氯己定（CHX）的抗菌效果。将牙齿用0.1 mL粪肠球菌（3×10⁸细胞/mL）污染，并随机分为八个治疗组：第1组（2.5%次氯酸钠）；第2组（PDT）；第3组（2% CHX）；第4组（TAM）；第5组（蜂胶），第6组（臭氧），第7组（阳性对照）和第8组（阴性对照）。治疗后，采集根管内容物样本。将样本在血琼脂平板上一式三份培养，以确定菌落形成单位（CFU）/mL的数量。通过扫描电子显微镜（SEM）分析牙齿，以确定有污染和碎屑区域的百分比。CFU/mL计数最低的组是臭氧组，其值与PDT组相似。SEM图像显示，2.5%次氯酸钠组的污染区域百分比最低。PDT、2% CHX、TAM、蜂胶和臭氧的应用显示出与2.5 %次氯酸钠相似的抗菌潜力。
