Yusenko Maria V, Zubakov Dmitry, Kovacs Gyula
Laboratory of Molecular Oncology, Medical Faculty, Ruprecht-Karls University, Heidelberg, Germany.
Int J Biol Sci. 2009 Jul 29;5(6):517-27. doi: 10.7150/ijbs.5.517.
Due to overlapping morphology, malignant chromophobe renal cell carcinomas (RCC) and benign renal oncocytomas (RO) may pose a diagnostic problem. In the present study, we have applied different algorithms to evaluate the data sets obtained by hybridisation of pooled and also individual samples of renal cell tumours (RCT) onto two different gene expression platforms. The two approaches revealed high similarities in the gene expression profiles of chromophobe RCCs and ROs but also some differences. After identifying the differentially expressed genes by statistic analyses, the candidate genes were further selected by a real time and normal RT-PCR and their products were analysed by immunohistochemistry. We have identified CD82 and S100A1 as valuable markers for chromophobe RCC as well as AQP6 for ROs. However, these genes are expressed at the protein level in other types of RCTs as well albeit at a low frequency and low intensity. As none of the selected genes marks exclusively one type of RCTs, for the differential diagnosis of chromophobe RCCs and ROs, a set of markers such as CD82, S100A1 and AQP6 as well as some others would be an option in routine histological laboratories.
由于形态学上的重叠，恶性嫌色性肾细胞癌（RCC）和良性肾嗜酸细胞瘤（RO）可能会带来诊断难题。在本研究中，我们应用了不同算法来评估通过将肾细胞肿瘤（RCT）的混合样本以及单个样本与两种不同基因表达平台杂交而获得的数据集。这两种方法揭示了嫌色性RCC和RO的基因表达谱有高度相似性，但也存在一些差异。通过统计分析确定差异表达基因后，通过实时和常规RT-PCR进一步筛选候选基因，并通过免疫组织化学分析其产物。我们已确定CD82和S100A1是嫌色性RCC的有价值标志物，而AQP6是RO的标志物。然而，这些基因在其他类型的RCT中也有蛋白水平的表达，尽管频率低且强度弱。由于所选基因均不能唯一标记一种类型的RCT，对于嫌色性RCC和RO的鉴别诊断，在常规组织学实验室中，一组标志物如CD82、S100A1和AQP6以及其他一些标志物可能是一种选择。
