Medical Mycology Research Center, Chiba University, Japan 1-8-1 Inohana, Chuo-ku, Chiba, 260-8673, Japan.
Mycopathologia. 2010 Jan;169(1):47-55. doi: 10.1007/s11046-009-9230-8.
We applied multilocus microsatellite typing (MLMT) method to investigate the genetic relation between Cryptococcus neoformans var. grubii clinical and environmental isolates in São Paulo, Brazil. This MLMT method includes three functional gene sequences of C. neoformans var. grubii, which are dispersed on three chromosomes. In all, 89 strains (36 clinical and 53 environmental isolates) were analyzed. Of 36 clinical strains, 20 belonged to a major type of MLMT-13 (55.6%). They were mainly isolated from clinical specimens. About 52.8% of strains from the environment belong to a major type of MLMT-36, which are indigenous to environments and which were not isolated from clinical samples. Thus, we recognized two genotypes that distinguish majority of clinical and environmental strains. No differences were found in antifungal susceptibility and capsule size between major environmental and clinical MLMT types.
我们应用多位点微卫星分型（MLMT）方法来研究巴西圣保罗的新型隐球菌格卢变种临床和环境分离株之间的遗传关系。该 MLMT 方法包括新型隐球菌格卢变种的三个功能基因序列，这些序列分散在三个染色体上。总共分析了 89 株（36 株临床株和 53 株环境分离株）。在 36 株临床株中，20 株属于 MLMT-13 的主要类型（55.6%）。它们主要从临床标本中分离得到。约 52.8%的环境株属于 MLMT-36 的主要类型，这些是环境中的本土株，并非从临床样本中分离得到。因此，我们识别出两种主要的基因型，它们可以区分大多数临床和环境分离株。主要的环境和临床 MLMT 类型之间的抗真菌敏感性和荚膜大小没有差异。
