中国和日本某变种特定基因型的优势地位。
The Predominance of a Specific Genotype of var. in China and Japan.
作者信息
Zhu Jian, Cao Jiafei, Li Tingying, Liu Qi, Kang Yingqian, Chen Zhenghong, Meyer Wieland, Mikami Yuzuru, Long Mei
机构信息
Department of Gastroenterology, Guizhou Provincial Orthopedic Hospital, Guiyang, Guizhou 550014, China.
Department of Gastroenterology, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550004, China.
出版信息
Comput Math Methods Med. 2022 Aug 5;2022:8162096. doi: 10.1155/2022/8162096. eCollection 2022.
BACKGROUND
The extensive deployment of molecular genotyping methods is the top reliable keyword to characterize the population genetic structure of in the past decade. However, studies involving genotypic analysis of var. from China and Japan are limited.
OBJECTIVES
We address this challenge to determine the genotype distribution of var. strains from China and Japan.
METHODS
Genotypic analysis of 52 var. isolates was performed using multilocus microsatellite typing (MLMT) based on the sequence analysis of 3 functional genes. In order to place the herein-studied strains into the global picture, 22 strains randomly selected from the 52 strains studied by MLMT were also analyzed by restriction fragment length polymorphism analysis of the orotidine monophosphate pyrophosphorylase gene (-RFLP), M13 PCR-fingerprinting, and multilocus sequence typing (MLST).
RESULTS
MLMT classified 46 (88.5%) of the 52 strains as genotype MLMT-17. The high prevalence of the MLMT-17 type was observed for environmental and clinical isolates from China and Japan. -RFLP analysis, M13 PCR-fingerprinting, and MLST showed that most of these belong to the VNI/ST5 (M5) genotype.
CONCLUSIONS
Our study suggests the predominance of a specific genotype of var. in China and Japan.
背景
在过去十年中，分子基因分型方法的广泛应用是描述[具体研究对象]群体遗传结构的首要可靠关键词。然而，涉及来自中国和日本的[具体品种]基因型分析的研究有限。
目的
我们应对这一挑战，以确定来自中国和日本的[具体品种]菌株的基因型分布。
方法
基于3个功能基因的序列分析，使用多位点微卫星分型（MLMT）对52株[具体品种]分离株进行基因型分析。为了将本文研究的菌株置于全球背景下，还通过乳清苷酸单磷酸焦磷酸化酶基因（-RFLP）的限制性片段长度多态性分析、M13 PCR指纹图谱分析和多位点序列分型（MLST）对从52株经MLMT研究的菌株中随机选择的22株进行了分析。
结果
MLMT将52株菌株中的46株（88.5%）分类为基因型MLMT-17。在中国和日本的环境和临床分离株中均观察到MLMT-17型的高流行率。-RFLP分析、M13 PCR指纹图谱分析和MLST表明，其中大多数属于VNI/ST5（M5）基因型。
结论
我们的研究表明，在中国和日本，[具体品种]的一种特定基因型占主导地位。
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